Human GTF2B ELISA Kit

Alternative name
Human general transcription factor TFIIB ELISA Kit;Human S300-II ELISA Kit;Human TF2B ELISA Kit;Human TFIIB ELISA Kit;Human general transcription factor IIB ELISA Kit;Human transcription initiation factor IIB ELISA Kit;Human RNA polymerase II transcription factor IIB ELISA Kit;
Catalog
E017679
species
Human
GeneGTF2B
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human GTF2B. No significant cross-reactivity or interference between Human GTF2B and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human GTF2B and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman GTF2B ELISA Kit allows for the in vitro quantitative determination of GTF2B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of GTF2B in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GTF2B. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human GTF2B and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GTF2B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human GTF2B. You can calculate the concentration of Human GTF2B in the samples by comparing the OD of the samples to the standard curve.
Human Transcription initiation factor IIB Protein information
Uniprot ID TF2B_HUMAN
Uniprot AC Q00403; A8K1A7; Q5JS30;
UniGene Hs.481852;
GeneID 2959
KEGG hsa:2959;
Human Transcription initiation factor IIB Protein SEQUENCE
SEQUENCE 316 AA; 34833 MW; 9CC7E102526C2722 CRC64;
MASTSRLDAL PRVTCPNHPD AILVEDYRAG DMICPECGLV VGDRVIDVGS
EWRTFSNDKA TKDPSRVGDS QNPLLSDGDL STMIGKGTGA ASFDEFGNSK
YQNRRTMSSS DRAMMNAFKE ITTMADRINL PRNIVDRTNN LFKQVYEQKS
LKGRANDAIA SACLYIACRQ EGVPRTFKEI CAVSRISKKE IGRCFKLILK
ALETSVDLIT TGDFMSRFCS NLCLPKQVQM AATHIARKAV ELDLVPGRSP
ISVAAAAIYM ASQASAEKRT QKEIGDIAGV ADVTIRQSYR LIYPRAPDLF
PTDFKFDTPV DKLPQL
UCSC uc001dmo.5; human.;

Category

Human GTF2B ELISA Kit

Human general transcription factor TFIIB ELISA Kit;Human S300-II ELISA Kit;Human TF2B ELISA Kit;Human TFIIB ELISA Kit;Human general transcription factor IIB ELISA Kit;Human transcription initiation factor IIB ELISA Kit;Human RNA polymerase II transcription factor IIB ELISA Kit;