Human HRAS ELISA Kit

Alternative name
Human H-Ras-1 ELISA Kit;Human Ha-Ras ELISA Kit;Human transforming protein p21 ELISA Kit;Human C-H-RAS ELISA Kit;Human p21ras ELISA Kit;Human HRAS1 ELISA Kit;Human C-BAS/HAS ELISA Kit;Human C-HA-RAS1 ELISA Kit;Human CTLO ELISA Kit;Human H-RASIDX ELISA Kit;Human HAMSV ELISA Kit;Human RASH1 ELISA Kit;Human HRas proto-oncogene, GTPase ELISA Kit;Human GTPase HRas ELISA Kit;Human GTP- and GDP-binding peptide B ELISA Kit;Human Ha-Ras1 proto-oncoprotein ELISA Kit;Human Harvey rat sarcoma viral oncogene homolog ELISA Kit;Human Harvey rat sarcoma viral oncoprotein ELISA Kit;Human Ras family small GTP binding protein H-Ras ELISA Kit;Human c-has/bas p21 protein ELISA Kit;Human c-ras-Ki-2 activated oncogene ELISA Kit;Human p19 H-RasIDX protein ELISA Kit;Human transformation gene: oncogene HAMSV ELISA Kit;Human v-Ha-ras Harvey rat sarcoma viral oncogene homolog ELISA Kit;
Catalog
E017704
species
Human
GeneHRAS
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.094ng/ml
Intended UseHuman HRAS ELISA Kit allows for the in vitro quantitative determination of HRAS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human HRAS antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human HRAS antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human HRAS amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human HRAS can be calculated.
Human GTPase HRas, N-terminally processed Protein information
Uniprot ID RASH_HUMAN
Uniprot AC P01112; B5BUA0; Q14080; Q6FHV9; Q9BR65; Q9UCE2;
UniGene Hs.37003;
GeneID 3265
KEGG hsa:3265;
Human GTPase HRas, N-terminally processed Protein SEQUENCE
SEQUENCE 189 AA; 21298 MW; EE6DC2D933E2856A CRC64;
MTEYKLVVVG AGGVGKSALT IQLIQNHFVD EYDPTIEDSY RKQVVIDGET
CLLDILDTAG QEEYSAMRDQ YMRTGEGFLC VFAINNTKSF EDIHQYREQI
KRVKDSDDVP MVLVGNKCDL AARTVESRQA QDLARSYGIP YIETSAKTRQ
GVEDAFYTLV REIRQHKLRK LNPPDESGPG CMSCKCVLS
UCSC uc010qvw.3; human. [P01112-1];

Category

Human HRAS ELISA Kit