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Human GNB3 ELISA Kit

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  • Alternative name

    Human transducin beta chain 3 ELISA Kit;Human CSNB1H ELISA Kit;Human G protein subunit beta 3 ELISA Kit;Human guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 ELISA Kit;Human G protein, beta-3 subunit ELISA Kit;Human GTP-binding regulatory protein beta-3 chain ELISA Kit;Human guanine nucleotide binding protein (G protein), beta polypeptide 3 ELISA Kit;Human guanine nucleotide-binding protein G(I)/G(S)/G(T) beta subunit 3 ELISA Kit;Human hypertension associated protein ELISA Kit;
  • Catalog
    E017760
  • species
    Human
  • GeneGNB3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.469ng/ml
  • Intended UseHuman GNB3 ELISA Kit allows for the in vitro quantitative determination of GNB3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GNB3 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GNB3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GNB3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GNB3 can be calculated.
  • Human Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 Protein information
  • Uniprot ID GBB3_HUMAN
  • Uniprot AC P16520; Q96B71; Q9BQC0;
  • UniGene Hs.631657;
  • GeneID 2784
  • KEGG hsa:2784;
  • Human Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3 Protein SEQUENCE

  • SEQUENCE 340 AA; 37221 MW; 896E706A61B8D74F CRC64;

    MGEMEQLRQE AEQLKKQIAD ARKACADVTL AELVSGLEVV GRVQMRTRRT

    LRGHLAKIYA MHWATDSKLL VSASQDGKLI VWDSYTTNKV HAIPLRSSWV

    MTCAYAPSGN FVACGGLDNM CSIYNLKSRE GNVKVSRELS AHTGYLSCCR

    FLDDNNIVTS SGDTTCALWD IETGQQKTVF VGHTGDCMSL AVSPDFNLFI

    SGACDASAKL WDVREGTCRQ TFTGHESDIN AICFFPNGEA ICTGSDDASC

    RLFDLRADQE LICFSHESII CGITSVAFSL SGRLLFAGYD DFNCNVWDSM

    KSERVGILSG HDNRVSCLGV TADGMAVATG SWDSFLKIWN

  • UCSC uc001qrd.3; human. [P16520-1];



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