Human HSPA12B ELISA Kit

Alternative name
Human C20orf60 ELISA Kit;Human heat shock protein family A (Hsp70) member 12B ELISA Kit;Human heat shock 70 kDa protein 12B ELISA Kit;
Catalog
E017949
species
Human
GeneHSPA12B
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HSPA12B. No significant cross-reactivity or interference between human HSPA12B and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HSPA12B is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseHuman HSPA12B ELISA Kit allows for the in vitro quantitative determination of HSPA12B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSPA12B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSPA12B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSPA12B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSPA12B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Heat shock 70 kDa protein 12B Protein information
Uniprot ID HS12B_HUMAN
Uniprot AC Q96MM6; D3DVX7; Q2TAK3; Q9BR52;
UniGene Hs.516854;
GeneID 116835
KEGG hsa:116835;
Human Heat shock 70 kDa protein 12B Protein SEQUENCE
SEQUENCE 686 AA; 75688 MW; 5F873AE97E366606 CRC64;
MLAVPEMGLQ GLYIGSSPER SPVPSPPGSP RTQESCGIAP LTPSQSPKPE
VRAPQQASFS VVVAIDFGTT SSGYAFSFAS DPEAIHMMRK WEGGDPGVAH
QKTPTCLLLT PEGAFHSFGY TARDYYHDLD PEEARDWLYF EKFKMKIHSA
TDLTLKTQLE AVNGKTMPAL EVFAHALRFF REHALQELRE QSPSLPEKDT
VRWVLTVPAI WKQPAKQFMR EAAYLAGLVS RENAEQLLIA LEPEAASVYC
RKLRLHQLLD LSGRAPGGGR LGERRSIDSS FRQAREQLRR SRHSRTFLVE
SGVGELWAEM QAGDRYVVAD CGGGTVDLTV HQLEQPHGTL KELYKASGGP
YGAVGVDLAF EQLLCRIFGE DFIATFKRQR PAAWVDLTIA FEARKRTAGP
HRAGALNISL PFSFIDFYRK QRGHNVETAL RRSSVNFVKW SSQGMLRMSC
EAMNELFQPT VSGIIQHIEA LLARPEVQGV KLLFLVGGFA ESAVLQHAVQ
AALGARGLRV VVPHDVGLTI LKGAVLFGQA PGVVRVRRSP LTYGVGVLNR
FVPGRHPPEK LLVRDGRRWC TDVFERFVAA EQSVALGEEV RRSYCPARPG
QRRVLINLYC CAAEDARFIT DPGVRKCGAL SLELEPADCG QDTAGAPPGR
REIRAAMQFG DTEIKVTAVD VSTNRSVRAS IDFLSN
UCSC uc002wjd.4; human.;

Category

Human HSPA12B ELISA Kit

Human C20orf60 ELISA Kit;Human heat shock protein family A (Hsp70) member 12B ELISA Kit;Human heat shock 70 kDa protein 12B ELISA Kit;