Human HSPA1L ELISA Kit

Alternative name
Human heat shock 70 kDa protein 1L ELISA Kit;Human heat shock 70 kDa protein 1-Hom ELISA Kit;Human HSP70-HOM ELISA Kit;Human HSP70-1L ELISA Kit;Human HSP70T ELISA Kit;Human hum70t ELISA Kit;Human heat shock protein family A (Hsp70) member 1 like ELISA Kit;Human heat shock 70 kDa protein 1-like ELISA Kit;Human heat shock 10kDa protein 1-like ELISA Kit;Human heat shock 70kD protein-like 1 ELISA Kit;Human heat shock 70kDa protein 1-like ELISA Kit;
Catalog
E017952
species
Human
GeneHSPA1L
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman HSPA1L ELISA Kit allows for the in vitro quantitative determination of HSPA1L , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalIntended Uses: This HSPA1L ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofHSPA1L in the sample, thisHSPA1L ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusHSPA1L concentration. The concentration ofHSPA1L in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific forHSPA1L. Standards or samples are then added to the microtiter plate wells andHSPA1L if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount ofHSPA1L present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific forHSPA1L are added to each well to "sandwich" theHSPA1L immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that containHSPA1L and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Human Heat shock 70 kDa protein 1-like Protein information
Uniprot ID HS71L_HUMAN
Uniprot AC P34931; A6NNB0; B0UXW8; O75634; Q2HXR3; Q8NE72; Q96QC9; Q9UQM1;
UniGene Hs.690634;
GeneID 3305
KEGG hsa:3305;
Human Heat shock 70 kDa protein 1-like Protein SEQUENCE
SEQUENCE 641 AA; 70375 MW; A9339D7657166FF7 CRC64;
MATAKGIAIG IDLGTTYSCV GVFQHGKVEI IANDQGNRTT PSYVAFTDTE
RLIGDAAKNQ VAMNPQNTVF DAKRLIGRKF NDPVVQADMK LWPFQVINEG
GKPKVLVSYK GENKAFYPEE ISSMVLTKLK ETAEAFLGHP VTNAVITVPA
YFNDSQRQAT KDAGVIAGLN VLRIINEPTA AAIAYGLDKG GQGERHVLIF
DLGGGTFDVS ILTIDDGIFE VKATAGDTHL GGEDFDNRLV SHFVEEFKRK
HKKDISQNKR AVRRLRTACE RAKRTLSSST QANLEIDSLY EGIDFYTSIT
RARFEELCAD LFRGTLEPVE KALRDAKMDK AKIHDIVLVG GSTRIPKVQR
LLQDYFNGRD LNKSINPDEA VAYGAAVQAA ILMGDKSEKV QDLLLLDVAP
LSLGLETAGG VMTALIKRNS TIPTKQTQIF TTYSDNQPGV LIQVYEGERA
MTKDNNLLGR FDLTGIPPAP RGVPQIEVTF DIDANGILNV TATDKSTGKV
NKITITNDKG RLSKEEIERM VLDAEKYKAE DEVQREKIAA KNALESYAFN
MKSVVSDEGL KGKISESDKN KILDKCNELL SWLEVNQLAE KDEFDHKRKE
LEQMCNPIIT KLYQGGCTGP ACGTGYVPGR PATGPTIEEV D
UCSC uc003nxh.4; human.;

Category

Human HSPA1L ELISA Kit