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Human HSPA1A ELISA Kit

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  • Alternative name

    Human Heat shock 70 kDa protein 1 ELISA Kit;Human HSP70-1 ELISA Kit;Human HSP70.1 ELISA Kit;Human HSPA1 ELISA Kit;Human HSX70 ELISA Kit;Human HSP70-1B ELISA Kit;Human HSP70-2 ELISA Kit;Human HSP70.2 ELISA Kit;Human heat shock protein family A (Hsp70) member 1B ELISA Kit;Human heat shock 70 kDa protein 1B ELISA Kit;Human HSP70-1/HSP70-2 ELISA Kit;Human HSP70.1/HSP70.2 ELISA Kit;Human heat shock 70 kDa protein 1/2 ELISA Kit;Human heat shock 70 kDa protein 1A/1B ELISA Kit;Human heat shock 70 kDa protein 2 ELISA Kit;Human heat shock 70kD protein 1B ELISA Kit;Human heat shock 70kDa protein 1B ELISA Kit;
  • Catalog
    E017961
  • species
    Human
  • GeneHSPA1A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Heat Shock 70kDa Protein 1A (HSPA1A). No significant cross-reactivity or interference between Heat Shock 70kDa Protein 1A (HSPA1A) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.61ng/mL.
  • Intended UseHuman HSPA1A ELISA Kit allows for the in vitro quantitative determination of HSPA1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 1A (HSPA1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 1A (HSPA1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 1A (HSPA1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock 70kDa Protein 1A (HSPA1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Heat shock 70 kDa protein 1A Protein information
  • Uniprot ID HS71A_HUMAN
  • Uniprot AC P0DMV8; B4E3B6; P08107; P19790; Q5JQI4; Q5SP17; Q9UQL9; Q9UQM0;
  • UniGene Hs.274402; Hs.702139; Hs.719966; Hs.743411;
  • GeneID 3304
  • KEGG hsa:3304;
  • Human Heat shock 70 kDa protein 1A Protein SEQUENCE

  • SEQUENCE 641 AA; 70052 MW; 78F513118C96DE66 CRC64;

    MAKAAAIGID LGTTYSCVGV FQHGKVEIIA NDQGNRTTPS YVAFTDTERL

    IGDAAKNQVA LNPQNTVFDA KRLIGRKFGD PVVQSDMKHW PFQVINDGDK

    PKVQVSYKGE TKAFYPEEIS SMVLTKMKEI AEAYLGYPVT NAVITVPAYF

    NDSQRQATKD AGVIAGLNVL RIINEPTAAA IAYGLDRTGK GERNVLIFDL

    GGGTFDVSIL TIDDGIFEVK ATAGDTHLGG EDFDNRLVNH FVEEFKRKHK

    KDISQNKRAV RRLRTACERA KRTLSSSTQA SLEIDSLFEG IDFYTSITRA

    RFEELCSDLF RSTLEPVEKA LRDAKLDKAQ IHDLVLVGGS TRIPKVQKLL

    QDFFNGRDLN KSINPDEAVA YGAAVQAAIL MGDKSENVQD LLLLDVAPLS

    LGLETAGGVM TALIKRNSTI PTKQTQIFTT YSDNQPGVLI QVYEGERAMT

    KDNNLLGRFE LSGIPPAPRG VPQIEVTFDI DANGILNVTA TDKSTGKANK

    ITITNDKGRL SKEEIERMVQ EAEKYKAEDE VQRERVSAKN ALESYAFNMK

    SAVEDEGLKG KISEADKKKV LDKCQEVISW LDANTLAEKD EFEHKRKELE

    QVCNPIISGL YQGAGGPGPG GFGAQGPKGG SGSGPTIEEV D




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