Human HSPA5 ELISA Kit

Alternative name
Human GRP-78 ELISA Kit;Human endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 ELISA Kit;Human Heat shock 70 kDa protein 5 ELISA Kit;Human immunoglobulin heavy chain-binding protein ELISA Kit;Human BIP ELISA Kit;Human GRP78 ELISA Kit;Human HEL-S-89n ELISA Kit;Human MIF2 ELISA Kit;Human heat shock protein family A (Hsp70) member 5 ELISA Kit;Human 78 kDa glucose-regulated protein ELISA Kit;Human epididymis secretory sperm binding protein Li 89n ELISA Kit;Human glucose-regulated protein, 78kDa ELISA Kit;Human heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) ELISA Kit;
Catalog
E017969
species
Human
GeneHSPA5
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human HSPA5. No significant cross-reactivity or interference between Human HSPA5 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.039 ng/ml
Intended UseHuman HSPA5 ELISA Kit allows for the in vitro quantitative determination of HSPA5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BIP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BIP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BIP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BIP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 78 kDa glucose-regulated protein Protein information
Uniprot ID GRP78_HUMAN
Uniprot AC P11021; B0QZ61; Q2EF78; Q9NPF1; Q9UK02;
UniGene Hs.743241;
GeneID 3309
KEGG hsa:3309;
Human 78 kDa glucose-regulated protein Protein SEQUENCE
SEQUENCE 654 AA; 72333 MW; 59B7D8D85BC32A00 CRC64;
MKLSLVAAML LLLSAARAEE EDKKEDVGTV VGIDLGTTYS CVGVFKNGRV
EIIANDQGNR ITPSYVAFTP EGERLIGDAA KNQLTSNPEN TVFDAKRLIG
RTWNDPSVQQ DIKFLPFKVV EKKTKPYIQV DIGGGQTKTF APEEISAMVL
TKMKETAEAY LGKKVTHAVV TVPAYFNDAQ RQATKDAGTI AGLNVMRIIN
EPTAAAIAYG LDKREGEKNI LVFDLGGGTF DVSLLTIDNG VFEVVATNGD
THLGGEDFDQ RVMEHFIKLY KKKTGKDVRK DNRAVQKLRR EVEKAKRALS
SQHQARIEIE SFYEGEDFSE TLTRAKFEEL NMDLFRSTMK PVQKVLEDSD
LKKSDIDEIV LVGGSTRIPK IQQLVKEFFN GKEPSRGINP DEAVAYGAAV
QAGVLSGDQD TGDLVLLDVC PLTLGIETVG GVMTKLIPRN TVVPTKKSQI
FSTASDNQPT VTIKVYEGER PLTKDNHLLG TFDLTGIPPA PRGVPQIEVT
FEIDVNGILR VTAEDKGTGN KNKITITNDQ NRLTPEEIER MVNDAEKFAE
EDKKLKERID TRNELESYAY SLKNQIGDKE KLGGKLSSED KETMEKAVEE
KIEWLESHQD ADIEDFKAKK KELEEIVQPI ISKLYGSAGP PPTGEEDTAE
KDEL

Category

Human HSPA5 ELISA Kit