Human HS6ST2 ELISA Kit

Alternative name
Human HS6ST-2 ELISA Kit;Human heparan sulfate 6-O-sulfotransferase 2 ELISA Kit;Human heparan-sulfate 6-O-sulfotransferase 2 ELISA Kit;
Catalog
E018266
species
Human
GeneHS6ST2
SpecificityThis assay has high sensitivity and excellent specificity for detection of Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2). No significant cross-reactivity or interference between Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.053ng/mL.
Intended UseHuman HS6ST2 ELISA Kit allows for the in vitro quantitative determination of HS6ST2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparan Sulfate-6-O-Sulfotransferase 2 (HS6ST2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Heparan-sulfate 6-O-sulfotransferase 2 Protein information
Uniprot ID H6ST2_HUMAN
Uniprot AC Q8N3T4; Q8NBN4; Q96SJ4;
UniGene Hs.385956;
GeneID 90161
KEGG hsa:90161;
Human Heparan-sulfate 6-O-sulfotransferase 2 Protein SEQUENCE
SEQUENCE 605 AA; 69130 MW; 10528AC424935B13 CRC64;
MALPACAVRE FEPPRQPERG APVRTTCPRR HSRVEAELAA SRPGSVAASV
RAGPPRGVSH GFHTRPLLDK PRKASSSLAG AACAPLFALL SRGRRRRMHV
LRRRWDLGSL CRALLTRGLA ALGHSLKHVL GAIFSKIFGP MASVGNMDEK
SNKLLLALVM LFLFAVIVLQ YVCPGTECQL LRLQAFSSPV PDPYRSEDES
SARFVPRYNF TRGDLLRKVD FDIKGDDLIV FLHIQKTGGT TFGRHLVRNI
QLEQPCECRV GQKKCTCHRP GKRETWLFSR FSTGWSCGLH ADWTELTSCV
PSVVDGKRDA RLRPSRNFHY ITILRDPVSR YLSEWRHVQR GATWKASLHV
CDGRPPTSEE LPSCYTGDDW SGCPLKEFMD CPYNLANNRQ VRMLSDLTLV
GCYNLSVMPE KQRNKVLLES AKSNLKHMAF FGLTEFQRKT QYLFEKTFNM
NFISPFTQYN TTRASSVEIN EEIQKRIEGL NFLDMELYSY AKDLFLQRYQ
FMRQKEHQEA RRKRQEQRKF LKGRLLQTHF QSQGQGQSQN PNQNQSQNPN
PNANQNLTQN LMQNLTQSLS QKENRESPKQ NSGKEQNDNT SNGTNDYIGS
VEKWR
UCSC uc011mvd.2; human. [Q96MM7-1];

Category

Human HS6ST2 ELISA Kit

Human HS6ST-2 ELISA Kit;Human heparan sulfate 6-O-sulfotransferase 2 ELISA Kit;Human heparan-sulfate 6-O-sulfotransferase 2 ELISA Kit;