Human HS6ST3 ELISA Kit

Alternative name
Human HS6ST-3 ELISA Kit;Human heparan sulfate 6-O-sulfotransferase 3 ELISA Kit;Human heparan-sulfate 6-O-sulfotransferase 3 ELISA Kit;
Catalog
E018268
species
Human
GeneHS6ST3
SpecificityThis assay has high sensitivity and excellent specificity for detection of HS6ST3. No significant cross-reactivity or interference between HS6ST3 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HS6ST3 is typically less than 0.073ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by ad
Intended UseHuman HS6ST3 ELISA Kit allows for the in vitro quantitative determination of HS6ST3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HS6ST3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HS6ST3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HS6ST3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HS6ST3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Heparan-sulfate 6-O-sulfotransferase 3 Protein information
Uniprot ID H6ST3_HUMAN
Uniprot AC Q8IZP7; Q5W0L0; Q68CW6;
UniGene Hs.171001; Hs.740393;
GeneID 266722
KEGG hsa:266722;
Human Heparan-sulfate 6-O-sulfotransferase 3 Protein SEQUENCE
SEQUENCE 471 AA; 54844 MW; 4A5F4DEA26FC7302 CRC64;
MDERFNKWLL TPVLTLLFVV IMYQYVSPSC TSSCTNFGEQ PRAGEAGPPA
VPGPARRAQA PPEEWERRPQ LPPPPRGPPE GPRGAAAPEE EDEEPGDPRE
GEEEEEEDEP DPEAPENGSL PRFVPRFNFS LKDLTRFVDF NIKGRDVIVF
LHIQKTGGTT FGRHLVKNIR LEQPCSCKAG QKKCTCHRPG KKETWLFSRF
STGWSCGLHA DWTELTNCVP AIMEKKDCPR NHSHTRNFYY ITMLRDPVSR
YLSEWKHVQR GATWKTSLHM CDGRSPTPDE LPTCYPGDDW SGVSLREFMD
CTYNLANNRQ VRMLADLSLV GCYNLTFMNE SERNTILLQS AKNNLKNMAF
FGLTEFQRKT QFLFERTFNL KFISPFTQFN ITRASNVEIN EGARQRIEDL
NFLDMQLYEY AKDLFQQRYH HTKQLEHQRD RQKRREERRL QREHRDHQWP
KEDGAAEGTV TEDYNSQVVR W
UCSC uc001vmw.4; human.;

Category

Human HS6ST3 ELISA Kit

Human HS6ST-3 ELISA Kit;Human heparan sulfate 6-O-sulfotransferase 3 ELISA Kit;Human heparan-sulfate 6-O-sulfotransferase 3 ELISA Kit;