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Alternative name
Heparanase ELISA KIT; Endo-glucoronidase ELISA KIT; Heparanase-1 ELISA KIT; Hpa1HPSE ELISA KIT; HEP ELISA KIT; HPA ELISA KIT; HPA1 ELISA KIT; HPR1 ELISA KIT; HPSE1 ELISA KIT; HSE1 ELISA KIT; Hpa1 ELISA KIT
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Catalog
E018276
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species
Human
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GeneHPA
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Heparanase (HPA).
No significant cross-reactivity or interference between Heparanase (HPA) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 11.9pg/mL.
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Intended UseHuman HPA ELISA Kit allows for the in vitro quantitative determination of HPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heparanase (HPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heparanase (HPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heparanase (HPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heparanase (HPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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