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Alternative name
Hepcidin ELISA KIT; Liver-expressed antimicrobial peptide 1 ELISA KIT; LEAP-1 ELISA KIT; Putative liver tumor regressor ELISA KIT; PLTRHAMP ELISA KIT; HEPC ELISA KIT; LEAP1 ELISA KIT; UNQ487/PRO1003 ELISA KIT; LEAP-1 ELISA KIT; PLTR ELISA KIT; Hepc25 ELISA KIT; Hepc20 ELISA KIT
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Catalog
E018418
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species
Human
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GeneHepc
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SpecificityThis assay has high sensitivity and excellent specificity for detection of HEPC. No significant cross-reactivity or interference between HEPC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HEPC and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman Hepc ELISA Kit allows for the in vitro quantitative determination of Hepc , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCardiovascular
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Product Description
specificalPrinciple of the assay: HEPC ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HEPC. Standards or samples are then added to the microtiter plate wells and HEPC if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HEPC present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HEPC are added to each well to "sandwich" the HEPC immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HEPC and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HEPC concentration in each sample is interpolated from this standard curve.
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