Human HK2 ELISA Kit

Alternative name
Human hexokinase type II ELISA Kit;Human HK II ELISA Kit;Human muscle form hexokinase ELISA Kit;Human HKII ELISA Kit;Human HXK2 ELISA Kit;Human hexokinase 2 ELISA Kit;Human hexokinase-2 ELISA Kit;Human hexokinase-2, muscle ELISA Kit;
Catalog
E018548
species
Human
GeneHK2
SpecificityThis assay has high sensitivity and excellent specificity for detection of HK2. No significant cross-reactivity or interference between HK2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HK2 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL
Intended UseHuman HK2 ELISA Kit allows for the in vitro quantitative determination of HK2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyHuman ELISA Kit
Product Description
specificalIntended Uses: This HK2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HK2. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: HK2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HK2 antibody and an HK2-HRP conjugate. The assay sample and buffer are incubated together with HK2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HK2 concentration since HK2 from samples and HK2-HRP conjugate compete for the anti-HK2 antibody binding site. Since the number of sites is limited, as more sites are occupied by HK2 from the sample, fewer sites are left to bind HK2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HK2 concentration in each sample is interpolated from this standard curve.
Human Hexokinase-2 Protein information
Uniprot ID HXK2_HUMAN
Uniprot AC P52789; D6W5J2; Q8WU87; Q9UN82;
UniGene Hs.406266; Hs.591588;
GeneID 3099
KEGG hsa:3099;
Human Hexokinase-2 Protein SEQUENCE
SEQUENCE 917 AA; 102380 MW; F17CE1938CF13880 CRC64;
MIASHLLAYF FTELNHDQVQ KVDQYLYHMR LSDETLLEIS KRFRKEMEKG
LGATTHPTAA VKMLPTFVRS TPDGTEHGEF LALDLGGTNF RVLWVKVTDN
GLQKVEMENQ IYAIPEDIMR GSGTQLFDHI AECLANFMDK LQIKDKKLPL
GFTFSFPCHQ TKLDESFLVS WTKGFKSSGV EGRDVVALIR KAIQRRGDFD
IDIVAVVNDT VGTMMTCGYD DHNCEIGLIV GTGSNACYME EMRHIDMVEG
DEGRMCINME WGAFGDDGSL NDIRTEFDQE IDMGSLNPGK QLFEKMISGM
YMGELVRLIL VKMAKEELLF GGKLSPELLN TGRFETKDIS DIEGEKDGIR
KAREVLMRLG LDPTQEDCVA THRICQIVST RSASLCAATL AAVLQRIKEN
KGEERLRSTI GVDGSVYKKH PHFAKRLHKT VRRLVPGCDV RFLRSEDGSG
KGAAMVTAVA YRLADQHRAR QKTLEHLQLS HDQLLEVKRR MKVEMERGLS
KETHASAPVK MLPTYVCATP DGTEKGDFLA LDLGGTNFRV LLVRVRNGKW
GGVEMHNKIY AIPQEVMHGT GDELFDHIVQ CIADFLEYMG MKGVSLPLGF
TFSFPCQQNS LDESILLKWT KGFKASGCEG EDVVTLLKEA IHRREEFDLD
VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE
GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL
GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV
RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG
LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG
AALITAVACR IREAGQR
UCSC uc002snd.4; human.;

Category

Human HK2 ELISA Kit

Human hexokinase type II ELISA Kit;Human HK II ELISA Kit;Human muscle form hexokinase ELISA Kit;Human HKII ELISA Kit;Human HXK2 ELISA Kit;Human hexokinase 2 ELISA Kit;Human hexokinase-2 ELISA Kit;Human hexokinase-2, muscle ELISA Kit;