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Human HEXB ELISA Kit

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  • Alternative name

    Human beta-N-acetylhexosaminidase subunit beta ELISA Kit;Human hexosaminidase subunit B ELISA Kit;Human cervical cancer proto-oncogene 7 protein ELISA Kit;Human HCC-7 ELISA Kit;Human N-acetyl-beta-glucosaminidase subunit beta ELISA Kit;Human ENC-1AS ELISA Kit;Human HEL-248 ELISA Kit;Human HEL-S-111 ELISA Kit;Human hexosaminidase subunit beta ELISA Kit;Human beta-hexosaminidase subunit beta ELISA Kit;Human beta-hexosaminidase beta-subunit ELISA Kit;Human epididymis luminal protein 248 ELISA Kit;Human epididymis secretory protein Li 111 ELISA Kit;Human hexosaminidase B (beta polypeptide) ELISA Kit;
  • Catalog
    E018562
  • species
    Human
  • GeneHEXB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hexosaminidase B Beta (HEXb). No significant cross-reactivity or interference between Hexosaminidase B Beta (HEXb) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman HEXB ELISA Kit allows for the in vitro quantitative determination of HEXB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hexosaminidase B Beta (HEXb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hexosaminidase B Beta (HEXb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hexosaminidase B Beta (HEXb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hexosaminidase B Beta (HEXb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Beta-hexosaminidase subunit beta chain A Protein information
  • Uniprot ID HEXB_HUMAN
  • Uniprot AC P07686;
  • UniGene Hs.69293;
  • GeneID 3074
  • KEGG hsa:3074;
  • Human Beta-hexosaminidase subunit beta chain A Protein SEQUENCE

  • SEQUENCE 556 AA; 63111 MW; B3A0A36594F62536 CRC64;

    MELCGLGLPR PPMLLALLLA TLLAAMLALL TQVALVVQVA EAARAPSVSA

    KPGPALWPLP LSVKMTPNLL HLAPENFYIS HSPNSTAGPS CTLLEEAFRR

    YHGYIFGFYK WHHEPAEFQA KTQVQQLLVS ITLQSECDAF PNISSDESYT

    LLVKEPVAVL KANRVWGALR GLETFSQLVY QDSYGTFTIN ESTIIDSPRF

    SHRGILIDTS RHYLPVKIIL KTLDAMAFNK FNVLHWHIVD DQSFPYQSIT

    FPELSNKGSY SLSHVYTPND VRMVIEYARL RGIRVLPEFD TPGHTLSWGK

    GQKDLLTPCY SRQNKLDSFG PINPTLNTTY SFLTTFFKEI SEVFPDQFIH

    LGGDEVEFKC WESNPKIQDF MRQKGFGTDF KKLESFYIQK VLDIIATINK

    GSIVWQEVFD DKAKLAPGTI VEVWKDSAYP EELSRVTASG FPVILSAPWY

    LDLISYGQDW RKYYKVEPLD FGGTQKQKQL FIGGEACLWG EYVDATNLTP

    RLWPRASAVG ERLWSSKDVR DMDDAYDRLT RHRCRMVERG IAAQPLYAGY

    CNHENM

  • UCSC uc003kdf.4; human.;



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