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Alternative name
High affinity nerve growth factor receptor ELISA KIT; Neurotrophic tyrosine kinase receptor type 1 ELISA KIT; TRK1-transforming tyrosine kinase protein ELISA KIT; Tropomyosin-related kinase A ELISA KIT; Tyrosine kinase receptor ELISA KIT; Tyrosine kinase receptor A ELISA KIT; Trk-A ELISA KIT; gp140trk ELISA KIT; p140-TrkANTRK1 ELISA KIT; MTC ELISA KIT; TRK ELISA KIT; TRKA ELISA KIT; Trk-A ELISA KIT
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Catalog
E018590
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species
Human
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GeneTrkA/NTRK1
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<46.9pg/ml
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Intended UseHuman TrkA/NTRK1 ELISA Kit allows for the in vitro quantitative determination of TrkA/NTRK1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TrkA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TrkA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TrkA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TrkA can be calculated.
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