Human HRH4 ELISA Kit

Alternative name
Human H4R ELISA Kit;Human HH4R ELISA Kit;Human AXOR35 ELISA Kit;Human G-protein coupled receptor 105 ELISA Kit;Human GPRv53 ELISA Kit;Human pfi-013 ELISA Kit;Human SP9144 ELISA Kit;Human GPCR105 ELISA Kit;Human BG26 ELISA Kit;Human H4 ELISA Kit;Human histamine receptor H4 ELISA Kit;Human histamine H4 receptor ELISA Kit;
Catalog
E018691
species
Human
GeneHRH4
SpecificityThis assay has high sensitivity and excellent specificity for detection of Histamine Receptor H4 (HRH4). No significant cross-reactivity or interference between Histamine Receptor H4 (HRH4) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL.
Intended UseHuman HRH4 ELISA Kit allows for the in vitro quantitative determination of HRH4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histamine Receptor H4 (HRH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Histamine Receptor H4 (HRH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Histamine Receptor H4 (HRH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Histamine Receptor H4 (HRH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Histamine H4 receptor Protein information
Uniprot ID HRH4_HUMAN
Uniprot AC Q9H3N8; B0YJ19; B2KJ48; Q4G0I6; Q9GZQ0;
UniGene Hs.287388;
GeneID 59340
KEGG hsa:59340;
Human Histamine H4 receptor Protein SEQUENCE
SEQUENCE 390 AA; 44496 MW; C986B8AE7FF912C3 CRC64;
MPDTNSTINL SLSTRVTLAF FMSLVAFAIM LGNALVILAF VVDKNLRHRS
SYFFLNLAIS DFFVGVISIP LYIPHTLFEW DFGKEICVFW LTTDYLLCTA
SVYNIVLISY DRYLSVSNAV SYRTQHTGVL KIVTLMVAVW VLAFLVNGPM
ILVSESWKDE GSECEPGFFS EWYILAITSF LEFVIPVILV AYFNMNIYWS
LWKRDHLSRC QSHPGLTAVS SNICGHSFRG RLSSRRSLSA STEVPASFHS
ERQRRKSSLM FSSRTKMNSN TIASKMGSFS QSDSVALHQR EHVELLRARR
LAKSLAILLG VFAVCWAPYS LFTIVLSFYS SATGPKSVWY RIAFWLQWFN
SFVNPLLYPL CHKRFQKAFL KIFCIKKQPL PSQHSRSVSS
UCSC uc002kvi.3; human. [Q9H3N8-1];

Category

Human HRH4 ELISA Kit