Human HLA-DRA ELISA Kit

Alternative name
Human MHC class II antigen DRA ELISA Kit;Human HLA-DRA1 ELISA Kit;Human major histocompatibility complex, class II, DR alpha ELISA Kit;Human HLA class II histocompatibility antigen, DR alpha chain ELISA Kit;Human histocompatibility antigen HLA-DR alpha ELISA Kit;
Catalog
E018862
species
Human
GeneHLA-DRA
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HLA-DRA. No significant cross-reactivity or interference between human HLA-DRA and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HLA-DRA is typically less than 4.68 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
Intended UseHuman HLA-DRA ELISA Kit allows for the in vitro quantitative determination of HLA-DRA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HLA-DRA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HLA-DRA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HLA-DRA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HLA-DRA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human HLA class II histocompatibility antigen, DR alpha chain Protein information
Uniprot ID DRA_HUMAN
Uniprot AC P01903; A2BET4; Q30160; Q6IAZ1; Q861I2; Q9TP70;
UniGene Hs.520048;
GeneID 3122
KEGG hsa:3122;
Human HLA class II histocompatibility antigen, DR alpha chain Protein SEQUENCE
SEQUENCE 254 AA; 28607 MW; 3CD1CDBA952B2350 CRC64;
MAISGVPVLG FFIIAVLMSA QESWAIKEEH VIIQAEFYLN PDQSGEFMFD
FDGDEIFHVD MAKKETVWRL EEFGRFASFE AQGALANIAV DKANLEIMTK
RSNYTPITNV PPEVTVLTNS PVELREPNVL ICFIDKFTPP VVNVTWLRNG
KPVTTGVSET VFLPREDHLF RKFHYLPFLP STEDVYDCRV EHWGLDEPLL
KHWEFDAPSP LPETTENVVC ALGLTVGLVG IIIGTIFIIK GVRKSNAAER
RGPL
UCSC uc003obh.5; human.;

Category

Human HLA-DRA ELISA Kit

Human MHC class II antigen DRA ELISA Kit;Human HLA-DRA1 ELISA Kit;Human major histocompatibility complex, class II, DR alpha ELISA Kit;Human HLA class II histocompatibility antigen, DR alpha chain ELISA Kit;Human histocompatibility antigen HLA-DR alpha ELISA Kit;