Human HMGCS1 ELISA Kit

Alternative name
Human HMG-CoA synthase ELISA Kit;Human 3-hydroxy-3-methylglutaryl coenzyme A synthase ELISA Kit;Human HMGCS ELISA Kit;Human 3-hydroxy-3-methylglutaryl-CoA synthase 1 ELISA Kit;Human hydroxymethylglutaryl-CoA synthase, cytoplasmic ELISA Kit;Human 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase ELISA Kit;Human 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble) ELISA Kit;Human 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble) ELISA Kit;
Catalog
E018875
species
Human
GeneHMGCS1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human HMGCS1. No significant cross-reactivity or interference between Human HMGCS1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human HMGCS1 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseHuman HMGCS1 ELISA Kit allows for the in vitro quantitative determination of HMGCS1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HMGCS1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HMGCS1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human HMGCS1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HMGCS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human HMGCS1. You can calculate the concentration of Human HMGCS1 in the samples by comparing the OD of the samples to the standard curve.
Human Hydroxymethylglutaryl-CoA synthase, cytoplasmic Protein information
Uniprot ID HMCS1_HUMAN
Uniprot AC Q01581; B2RDL8;
UniGene Hs.397729; Hs.741287;
GeneID 3157
KEGG hsa:3157;
Human Hydroxymethylglutaryl-CoA synthase, cytoplasmic Protein SEQUENCE
SEQUENCE 520 AA; 57294 MW; C669212BF86CFF9B CRC64;
MPGSLPLNAE ACWPKDVGIV ALEIYFPSQY VDQAELEKYD GVDAGKYTIG
LGQAKMGFCT DREDINSLCM TVVQNLMERN NLSYDCIGRL EVGTETIIDK
SKSVKTNLMQ LFEESGNTDI EGIDTTNACY GGTAAVFNAV NWIESSSWDG
RYALVVAGDI AVYATGNARP TGGVGAVALL IGPNAPLIFE RGLRGTHMQH
AYDFYKPDML SEYPIVDGKL SIQCYLSALD RCYSVYCKKI HAQWQKEGND
KDFTLNDFGF MIFHSPYCKL VQKSLARMLL NDFLNDQNRD KNSIYSGLEA
FGDVKLEDTY FDRDVEKAFM KASSELFSQK TKASLLVSNQ NGNMYTSSVY
GSLASVLAQY SPQQLAGKRI GVFSYGSGLA ATLYSLKVTQ DATPGSALDK
ITASLCDLKS RLDSRTGVAP DVFAENMKLR EDTHHLVNYI PQGSIDSLFE
GTWYLVRVDE KHRRTYARRP TPNDDTLDEG VGLVHSNIAT EHIPSPAKKV
PRLPATAAEP EAAVISNGEH
UCSC uc003jnq.6; human.;

Category

Human HMGCS1 ELISA Kit