Human HAP1 ELISA Kit

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  • Alternative name

    Human HAP-1 ELISA Kit;Human neuroan 1 ELISA Kit;Human HAP2 ELISA Kit;Human HLP1 ELISA Kit;Human HIP5 ELISA Kit;Human HLP ELISA Kit;Human hHLP1 ELISA Kit;Human huntingtin associated protein 1 ELISA Kit;Human huntingtin-associated protein 1 ELISA Kit;Human huntingtin-associated protein 2 ELISA Kit;

  • Catalog
    E019042
  • species
    Human
  • GeneHAP1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Huntingtin Associated Protein 1 (HAP1). No significant cross-reactivity or interference between Huntingtin Associated Protein 1 (HAP1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.113ng/mL.
  • Intended UseHuman HAP1 ELISA Kit allows for the in vitro quantitative determination of HAP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Huntingtin Associated Protein 1 (HAP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Huntingtin Associated Protein 1 (HAP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Huntingtin Associated Protein 1 (HAP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Huntingtin Associated Protein 1 (HAP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Huntingtin-associated protein 1 Protein information
  • Uniprot ID HAP1_HUMAN
  • Uniprot AC P54257; A8MQB5; O75358; Q59GK4; Q9H4G3; Q9HA98; Q9NY90;
  • UniGene Hs.158300;
  • GeneID 9001
  • KEGG hsa:9001;
  • Human Huntingtin-associated protein 1 Protein SEQUENCE
  • SEQUENCE 671 AA; 75506 MW; 10971B807941B4EE CRC64;

    MRPKRLGRCC AGSRLGPGDP AALTCAPSPS ASPAPEPSAQ PQARGTGQRV

    GSRATSGSQF LSEARTGARP ASEAGAKAGA RRPSAFSAIQ GDVRSMPDNS

    DAPWTRFVFQ GPFGSRATGR GTGKAAGIWK TPAAYVGRRP GVSGPERAAF

    IRELEEALCP NLPPPVKKIT QEDVKVMLYL LEELLPPVWE SVTYGMVLQR

    ERDLNTAARI GQSLVKQNSV LMEENSKLEA LLGSAKEEIL YLRHQVNLRD

    ELLQLYSDSD EEDEDEEEEE EEKEAEEEQE EEEAEEDLQC AHPCDAPKLI

    SQEALLHQHH CPQLEALQEK LRLLEEENHQ LREEASQLDT LEDEEQMLIL

    ECVEQFSEAS QQMAELSEVL VLRLENYERQ QQEVARLQAQ VLKLQQRCRM

    YGAETEKLQK QLASEKEIQM QLQEESVWVG SQLQDLREKY MDCGGMLIEM

    QEEVKTLRQQ PPVSTGSATH YPYSVPLETL PGFQETLAEE LRTSLRRMIS

    DPVYFMERNY EMPRGDTSSL RYDFRYSEDR EQVRGFEAEE GLMLAADIMR

    GEDFTPAEEF VPQEELGAAK KVPAEEGVME EAELVSEETE GWEEVELELD

    EATRMNVVTS ALEASGLGPS HLDMNYVLQQ LANWQDAHYR RQLRWKMLQK

    GECPHGALPA ASRTSCRSSC R

  • UCSC uc002hxm.2; human. [P54257-1];



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