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Human HAPLN1 ELISA Kit

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  • Alternative name

    Human cartilage-linking protein 1 ELISA Kit;Human cartilage-link protein ELISA Kit;Human proteoglycan link protein ELISA Kit;Human CRTL1 ELISA Kit;Human CRT1 ELISA Kit;Human hyaluronan and proteoglycan link protein 1 ELISA Kit;Human Cartilage link protein ELISA Kit;
  • Catalog
    E019047
  • species
    Human
  • GeneHAPLN1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HAPLN1. No significant cross-reactivity or interference between HAPLN1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between HAPLN1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman HAPLN1 ELISA Kit allows for the in vitro quantitative determination of HAPLN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical    Priciple of the assay: HAPLN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HAPLN1 antibody and an HAPLN1-HRP conjugate. The assay sample and buffer are incubated together with HAPLN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HAPLN1 concentration since HAPLN1 from samples and HAPLN1-HRP conjugate compete for the anti-HAPLN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by HAPLN1 from the sample, fewer sites are left to bind HAPLN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HAPLN1 concentration in each sample is interpolated from this standard curve.
  • Human Hyaluronan and proteoglycan link protein 1 Protein information
  • Uniprot ID HPLN1_HUMAN
  • Uniprot AC P10915; B2R9A9;
  • UniGene Hs.2799;
  • GeneID 1404
  • KEGG hsa:1404;
  • Human Hyaluronan and proteoglycan link protein 1 Protein SEQUENCE

  • SEQUENCE 354 AA; 40166 MW; 315C96EC3AC2626A CRC64;

    MKSLLLLVLI SICWADHLSD NYTLDHDRAI HIQAENGPHL LVEAEQAKVF

    SHRGGNVTLP CKFYRDPTAF GSGIHKIRIK WTKLTSDYLK EVDVFVSMGY

    HKKTYGGYQG RVFLKGGSDS DASLVITDLT LEDYGRYKCE VIEGLEDDTV

    VVALDLQGVV FPYFPRLGRY NLNFHEAQQA CLDQDAVIAS FDQLYDAWRG

    GLDWCNAGWL SDGSVQYPIT KPREPCGGQN TVPGVRNYGF WDKDKSRYDV

    FCFTSNFNGR FYYLIHPTKL TYDEAVQACL NDGAQIAKVG QIFAAWKILG

    YDRCDAGWLA DGSVRYPISR PRRRCSPTEA AVRFVGFPDK KHKLYGVYCF

    RAYN

  • UCSC uc003kin.4; human.;



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