Human HADHB ELISA Kit

Alternative name
Human TP-BETA ELISA Kit;Human acetyl-CoA acyltransferase ELISA Kit;Human beta-ketothiolase ELISA Kit;Human ECHB ELISA Kit;Human MSTP029 ELISA Kit;Human MTPB ELISA Kit;Human hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit ELISA Kit;Human trifunctional enzyme subunit beta, mitochondrial ELISA Kit;Human 2-enoyl-Coenzyme A (CoA) hydratase, beta subunit ELISA Kit;Human 3-ketoacyl-Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein, beta subunit ELISA Kit;Human hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit ELISA Kit;
Catalog
E019130
species
Human
GeneHADHB
SpecificityThis assay has high sensitivity and excellent specificity for detection of human HADHB. No significant cross-reactivity or interference between human HADHB and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human HADHB is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman HADHB ELISA Kit allows for the in vitro quantitative determination of HADHB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HADHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HADHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HADHB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HADHB bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 3-ketoacyl-CoA thiolase Protein information
Uniprot ID ECHB_HUMAN
Uniprot AC P55084; B2RB16; B4E2W0; O14969; Q53TA6; Q96C77; Q9H3F5; Q9T2V8;
UniGene Hs.515848;
GeneID 3032
KEGG hsa:3032;
Human 3-ketoacyl-CoA thiolase Protein SEQUENCE
SEQUENCE 474 AA; 51294 MW; A7B41C37BEC1E6AD CRC64;
MTILTYPFKN LPTASKWALR FSIRPLSCSS QLRAAPAVQT KTKKTLAKPN
IRNVVVVDGV RTPFLLSGTS YKDLMPHDLA RAALTGLLHR TSVPKEVVDY
IIFGTVIQEV KTSNVAREAA LGAGFSDKTP AHTVTMACIS ANQAMTTGVG
LIASGQCDVI VAGGVELMSD VPIRHSRKMR KLMLDLNKAK SMGQRLSLIS
KFRFNFLAPE LPAVSEFSTS ETMGHSADRL AAAFAVSRLE QDEYALRSHS
LAKKAQDEGL LSDVVPFKVP GKDTVTKDNG IRPSSLEQMA KLKPAFIKPY
GTVTAANSSF LTDGASAMLI MAEEKALAMG YKPKAYLRDF MYVSQDPKDQ
LLLGPTYATP KVLEKAGLTM NDIDAFEFHE AFSGQILANF KAMDSDWFAE
NYMGRKTKVG LPPLEKFNNW GGSLSLGHPF GATGCRLVMA AANRLRKEGG
QYGLVAACAA GGQGHAMIVE AYPK
UCSC uc002rgz.4; human. [P55084-1];

Category

Human HADHB ELISA Kit