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Human HMBS ELISA Kit

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  • Alternative name

    Human PBG-D ELISA Kit;Human hydroxymethylbilane synthase ELISA Kit;Human HMBS ELISA Kit;Human pre-uroporphyrinogen synthase ELISA Kit;Human PBGD ELISA Kit;Human UPS ELISA Kit;Human PORC ELISA Kit;Human porphobilinogen deaminase ELISA Kit;Human porphyria, acute; Chester type ELISA Kit;Human uroporphyrinogen I synthase ELISA Kit;Human uroporphyrinogen I synthetase ELISA Kit;
  • Catalog
    E019142
  • species
    Human
  • GeneHMBS
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of HMBS. No significant cross-reactivity or interference between HMBS and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human HMBS is typically less than 0.27ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addin
  • Intended UseHuman HMBS ELISA Kit allows for the in vitro quantitative determination of HMBS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMBS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HMBS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HMBS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of HMBS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Porphobilinogen deaminase Protein information
  • Uniprot ID HEM3_HUMAN
  • Uniprot AC P08397; A8K2L0; G3V1P4; G5EA58; P08396; Q16012;
  • UniGene Hs.82609;
  • GeneID 3145
  • KEGG hsa:3145;
  • Human Porphobilinogen deaminase Protein SEQUENCE

  • SEQUENCE 361 AA; 39330 MW; 8F2F6F4150F1AD7E CRC64;

    MSGNGNAAAT AEENSPKMRV IRVGTRKSQL ARIQTDSVVA TLKASYPGLQ

    FEIIAMSTTG DKILDTALSK IGEKSLFTKE LEHALEKNEV DLVVHSLKDL

    PTVLPPGFTI GAICKRENPH DAVVFHPKFV GKTLETLPEK SVVGTSSLRR

    AAQLQRKFPH LEFRSIRGNL NTRLRKLDEQ QEFSAIILAT AGLQRMGWHN

    RVGQILHPEE CMYAVGQGAL GVEVRAKDQD ILDLVGVLHD PETLLRCIAE

    RAFLRHLEGG CSVPVAVHTA MKDGQLYLTG GVWSLDGSDS IQETMQATIH

    VPAQHEDGPE DDPQLVGITA RNIPRGPQLA AQNLGISLAN LLLSKGAKNI

    LDVARQLNDA H

  • UCSC uc001puz.2; human. [P08397-1];



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