Human HIF1A ELISA Kit

Alternative name
Human HIF-1-alpha ELISA Kit;Human HIF1-ALPHA ELISA Kit;Human ARNT-interacting protein ELISA Kit;Human basic-helix-loop-helix-PAS protein MOP1 ELISA Kit;Human class E basic helix-loop-helix protein 78 ELISA Kit;Human bHLHe78 ELISA Kit;Human member of PAS protein 1 ELISA Kit;Human PAS domain-containing protein 8 ELISA Kit;Human MOP1 ELISA Kit;Human PASD8 ELISA Kit;Human HIF-1A ELISA Kit;Human HIF-1alpha ELISA Kit;Human HIF1 ELISA Kit;Human hypoxia inducible factor 1 alpha subunit ELISA Kit;Human hypoxia-inducible factor 1-alpha ELISA Kit;Human ARNT interacting protein ELISA Kit;Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) ELISA Kit;Human hypoxia-inducible factor 1 alpha isoform I.3 ELISA Kit;Human hypoxia-inducible factor1alpha ELISA Kit;Human member of PAS superfamily 1 ELISA Kit;
Catalog
E019183
species
Human
GeneHIF1A
SpecificityThis assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 1 Alpha (HIF1a). No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha (HIF1a) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL.
Intended UseHuman HIF1A ELISA Kit allows for the in vitro quantitative determination of HIF1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha (HIF1a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 1 Alpha (HIF1a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 1 Alpha (HIF1a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Hypoxia-inducible factor 1-alpha Protein information
Uniprot ID HIF1A_HUMAN
Uniprot AC Q16665; C0LZJ3; Q53XP6; Q96PT9; Q9UPB1;
UniGene Hs.597216; Hs.719495;
GeneID 3091
KEGG hsa:3091;
Human Hypoxia-inducible factor 1-alpha Protein SEQUENCE
SEQUENCE 826 AA; 92670 MW; ABD4F7DAA135BE2D CRC64;
MEGAGGANDK KKISSERRKE KSRDAARSRR SKESEVFYEL AHQLPLPHNV
SSHLDKASVM RLTISYLRVR KLLDAGDLDI EDDMKAQMNC FYLKALDGFV
MVLTDDGDMI YISDNVNKYM GLTQFELTGH SVFDFTHPCD HEEMREMLTH
RNGLVKKGKE QNTQRSFFLR MKCTLTSRGR TMNIKSATWK VLHCTGHIHV
YDTNSNQPQC GYKKPPMTCL VLICEPIPHP SNIEIPLDSK TFLSRHSLDM
KFSYCDERIT ELMGYEPEEL LGRSIYEYYH ALDSDHLTKT HHDMFTKGQV
TTGQYRMLAK RGGYVWVETQ ATVIYNTKNS QPQCIVCVNY VVSGIIQHDL
IFSLQQTECV LKPVESSDMK MTQLFTKVES EDTSSLFDKL KKEPDALTLL
APAAGDTIIS LDFGSNDTET DDQQLEEVPL YNDVMLPSPN EKLQNINLAM
SPLPTAETPK PLRSSADPAL NQEVALKLEP NPESLELSFT MPQIQDQTPS
PSDGSTRQSS PEPNSPSEYC FYVDSDMVNE FKLELVEKLF AEDTEAKNPF
STQDTDLDLE MLAPYIPMDD DFQLRSFDQL SPLESSSASP ESASPQSTVT
VFQQTQIQEP TANATTTTAT TDELKTVTKD RMEDIKILIA SPSPTHIHKE
TTSATSSPYR DTQSRTASPN RAGKGVIEQT EKSHPRSPNV LSVALSQRTT
VPEEELNPKI LALQNAQRKR KMEHDGSLFQ AVGIGTLLQQ PDDHAATTSL
SWKRVKGCKS SEQNGMEQKT IILIPSDLAC RLLGQSMDES GLPQLTSYDC
EVNAPIQGSR NLLQGEELLR ALDQVN
UCSC uc001xfq.3; human. [Q16665-1];

Category

Human HIF1A ELISA Kit