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Human HIF1AN ELISA Kit

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  • Alternative name

    Human factor inhibiting HIF-1 ELISA Kit;Human FIH-1 ELISA Kit;Human hypoxia-inducible factor asparagine hydroxylase ELISA Kit;Human FIH1 ELISA Kit;Human hypoxia inducible factor 1 alpha subunit inhibitor ELISA Kit;Human hypoxia-inducible factor 1-alpha inhibitor ELISA Kit;Human factor inhibiting HIF1 ELISA Kit;Human peptide-aspartate beta-dioxygenase ELISA Kit;
  • Catalog
    E019184
  • species
    Human
  • GeneHIF1AN
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN). No significant cross-reactivity or interference between Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 14.4pg/mL.
  • Intended UseHuman HIF1AN ELISA Kit allows for the in vitro quantitative determination of HIF1AN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Inducible Factor 1 Alpha Subunit Inhibitor (HIF1aN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Hypoxia-inducible factor 1-alpha inhibitor Protein information
  • Uniprot ID HIF1N_HUMAN
  • Uniprot AC Q9NWT6; D3DR69; Q5W147; Q969Q7; Q9NPV5;
  • UniGene Hs.500788;
  • GeneID 55662
  • KEGG hsa:55662;
  • Human Hypoxia-inducible factor 1-alpha inhibitor Protein SEQUENCE

  • SEQUENCE 349 AA; 40285 MW; 96A033BA7B3BD8C7 CRC64;

    MAATAAEAVA SGSGEPREEA GALGPAWDES QLRSYSFPTR PIPRLSQSDP

    RAEELIENEE PVVLTDTNLV YPALKWDLEY LQENIGNGDF SVYSASTHKF

    LYYDEKKMAN FQNFKPRSNR EEMKFHEFVE KLQDIQQRGG EERLYLQQTL

    NDTVGRKIVM DFLGFNWNWI NKQQGKRGWG QLTSNLLLIG MEGNVTPAHY

    DEQQNFFAQI KGYKRCILFP PDQFECLYPY PVHHPCDRQS QVDFDNPDYE

    RFPNFQNVVG YETVVGPGDV LYIPMYWWHH IESLLNGGIT ITVNFWYKGA

    PTPKRIEYPL KAHQKVAIMR NIEKMLGEAL GNPQEVGPLL NTMIKGRYN

  • UCSC uc001krj.5; human.;



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