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Human HYOU1 ELISA Kit

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  • Alternative name

    Human 150 kDa oxygen-regulated protein ELISA Kit;Human ORP-150 ELISA Kit;Human 170 kDa glucose-regulated protein ELISA Kit;Human GRP-170 ELISA Kit;Human Grp170 ELISA Kit;Human ORP150 ELISA Kit;Human HSP12A ELISA Kit;Human hypoxia up-regulated 1 ELISA Kit;Human hypoxia up-regulated protein 1 ELISA Kit;Human oxygen regulated protein (150kD) ELISA Kit;
  • Catalog
    E019192
  • species
    Human
  • GeneHYOU1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Hypoxia Up Regulated 1 (HYOU1). No significant cross-reactivity or interference between Hypoxia Up Regulated 1 (HYOU1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.059ng/mL.
  • Intended UseHuman HYOU1 ELISA Kit allows for the in vitro quantitative determination of HYOU1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hypoxia Up Regulated 1 (HYOU1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Hypoxia Up Regulated 1 (HYOU1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hypoxia Up Regulated 1 (HYOU1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hypoxia Up Regulated 1 (HYOU1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Hypoxia up-regulated protein 1 Protein information
  • Uniprot ID HYOU1_HUMAN
  • Uniprot AC Q9Y4L1; A8C1Z0; B7Z909; Q2I204; Q53H25;
  • UniGene Hs.277704;
  • GeneID 10525
  • KEGG hsa:10525;
  • Human Hypoxia up-regulated protein 1 Protein SEQUENCE

  • SEQUENCE 999 AA; 111335 MW; FCE0F292466AFAB9 CRC64;

    MADKVRRQRP RRRVCWALVA VLLADLLALS DTLAVMSVDL GSESMKVAIV

    KPGVPMEIVL NKESRRKTPV IVTLKENERF FGDSAASMAI KNPKATLRYF

    QHLLGKQADN PHVALYQARF PEHELTFDPQ RQTVHFQISS QLQFSPEEVL

    GMVLNYSRSL AEDFAEQPIK DAVITVPVFF NQAERRAVLQ AARMAGLKVL

    QLINDNTATA LSYGVFRRKD INTTAQNIMF YDMGSGSTVC TIVTYQMVKT

    KEAGMQPQLQ IRGVGFDRTL GGLEMELRLR ERLAGLFNEQ RKGQRAKDVR

    ENPRAMAKLL REANRLKTVL SANADHMAQI EGLMDDVDFK AKVTRVEFEE

    LCADLFERVP GPVQQALQSA EMSLDEIEQV ILVGGATRVP RVQEVLLKAV

    GKEELGKNIN ADEAAAMGAV YQAAALSKAF KVKPFVVRDA VVYPILVEFT

    REVEEEPGIH SLKHNKRVLF SRMGPYPQRK VITFNRYSHD FNFHINYGDL

    GFLGPEDLRV FGSQNLTTVK LKGVGDSFKK YPDYESKGIK AHFNLDESGV

    LSLDRVESVF ETLVEDSAEE ESTLTKLGNT ISSLFGGGTT PDAKENGTDT

    VQEEEESPAE GSKDEPGEQV ELKEEAEAPV EDGSQPPPPE PKGDATPEGE

    KATEKENGDK SEAQKPSEKA EAGPEGVAPA PEGEKKQKPA RKRRMVEEIG

    VELVVLDLPD LPEDKLAQSV QKLQDLTLRD LEKQEREKAA NSLEAFIFET

    QDKLYQPEYQ EVSTEEQREE ISGKLSAAST WLEDEGVGAT TVMLKEKLAE

    LRKLCQGLFF RVEERKKWPE RLSALDNLLN HSSMFLKGAR LIPEMDQIFT

    EVEMTTLEKV INETWAWKNA TLAEQAKLPA TEKPVLLSKD IEAKMMALDR

    EVQYLLNKAK FTKPRPRPKD KNGTRAEPPL NASASDQGEK VIPPAGQTED

    AEPISEPEKV ETGSEPGDTE PLELGGPGAE PEQKEQSTGQ KRPLKNDEL

  • UCSC uc031yhc.2; human. [Q9Y4L1-1];



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