Human IDS ELISA Kit

Alternative name
Human alpha-L-iduronate sulfate sulfatase ELISA Kit;Human idursulfase ELISA Kit;Human SIDS ELISA Kit;Human MPS2 ELISA Kit;Human iduronate 2-sulfatase ELISA Kit;Human iduronate 2-sulfatase 14 kDa chain ELISA Kit;Human iduronate 2-sulfatase 42 kDa chain ELISA Kit;
Catalog
E019225
species
Human
GeneIDS
SpecificityNatural and recombinant human IDS
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity Sample For quantitative detection of human IDS in cell culture supernates, serum and plasma
Intended UseHuman IDS ELISA Kit allows for the in vitro quantitative determination of IDS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: Human IDS ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IDS has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: S26-P550) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IDS is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IDS amount of sample captured in plate. Background: Iduronate 2-sulfatase (IDS) is a sulfatase enzyme associated with Hunter syndrome. It encodes a member of the sulfatase family of proteins. Iduronate 2-sulfatase is involved in the lysosomal degradation of the glycosaminoglycans heparan sulfate and dermatan sulfate. The encoded preproprotein is proteolytically processed to generate two polypeptide chains. Mutations in this gene are associated with the X-linked lysosomal storage disease mucopolysaccharidosis type II, also known as Hunter syndrome. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed.
Human Iduronate 2-sulfatase 14 kDa chain Protein information
Uniprot ID IDS_HUMAN
Uniprot AC P22304; D3DWT4; Q14604; Q9BRM3;
UniGene Hs.460960; Hs.6795;
GeneID 3423
KEGG hsa:3423;
Human Iduronate 2-sulfatase 14 kDa chain Protein SEQUENCE
SEQUENCE 550 AA; 61873 MW; EA1B713417280413 CRC64;
MPPPRTGRGL LWLGLVLSSV CVALGSETQA NSTTDALNVL LIIVDDLRPS
LGCYGDKLVR SPNIDQLASH SLLFQNAFAQ QAVCAPSRVS FLTGRRPDTT
RLYDFNSYWR VHAGNFSTIP QYFKENGYVT MSVGKVFHPG ISSNHTDDSP
YSWSFPPYHP SSEKYENTKT CRGPDGELHA NLLCPVDVLD VPEGTLPDKQ
STEQAIQLLE KMKTSASPFF LAVGYHKPHI PFRYPKEFQK LYPLENITLA
PDPEVPDGLP PVAYNPWMDI RQREDVQALN ISVPYGPIPV DFQRKIRQSY
FASVSYLDTQ VGRLLSALDD LQLANSTIIA FTSDHGWALG EHGEWAKYSN
FDVATHVPLI FYVPGRTASL PEAGEKLFPY LDPFDSASQL MEPGRQSMDL
VELVSLFPTL AGLAGLQVPP RCPVPSFHVE LCREGKNLLK HFRFRDLEED
PYLPGNPREL IAYSQYPRPS DIPQWNSDKP SLKDIKIMGY SIRTIDYRYT
VWVGFNPDEF LANFSDIHAG ELYFVDSDPL QDHNMYNDSQ GGDLFQLLMP
UCSC uc011mxe.3; human. [P22304-1];

Category

Human IDS ELISA Kit

Human alpha-L-iduronate sulfate sulfatase ELISA Kit;Human idursulfase ELISA Kit;Human SIDS ELISA Kit;Human MPS2 ELISA Kit;Human iduronate 2-sulfatase ELISA Kit;Human iduronate 2-sulfatase 14 kDa chain ELISA Kit;Human iduronate 2-sulfatase 42 kDa chain ELISA Kit;