Human IDUA ELISA Kit

Alternative name
Human IDA ELISA Kit;Human MPS1 ELISA Kit;Human iduronidase, alpha-L- ELISA Kit;Human alpha-L-iduronidase ELISA Kit;
Catalog
E019234
species
Human
GeneIDUA
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human IDUA. No significant cross-reactivity or interference between Human IDUA and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.039 ng/ml
Intended UseHuman IDUA ELISA Kit allows for the in vitro quantitative determination of IDUA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IDUA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IDUA and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IDUA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IDUA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alpha-L-iduronidase Protein information
Uniprot ID IDUA_HUMAN
Uniprot AC P35475; B3KWK6;
UniGene Hs.89560;
GeneID 3425
KEGG hsa:3425;
Human Alpha-L-iduronidase Protein SEQUENCE
SEQUENCE 653 AA; 72670 MW; 9D2399B22FD172BD CRC64;
MRPLRPRAAL LALLASLLAA PPVAPAEAPH LVHVDAARAL WPLRRFWRST
GFCPPLPHSQ ADQYVLSWDQ QLNLAYVGAV PHRGIKQVRT HWLLELVTTR
GSTGRGLSYN FTHLDGYLDL LRENQLLPGF ELMGSASGHF TDFEDKQQVF
EWKDLVSSLA RRYIGRYGLA HVSKWNFETW NEPDHHDFDN VSMTMQGFLN
YYDACSEGLR AASPALRLGG PGDSFHTPPR SPLSWGLLRH CHDGTNFFTG
EAGVRLDYIS LHRKGARSSI SILEQEKVVA QQIRQLFPKF ADTPIYNDEA
DPLVGWSLPQ PWRADVTYAA MVVKVIAQHQ NLLLANTTSA FPYALLSNDN
AFLSYHPHPF AQRTLTARFQ VNNTRPPHVQ LLRKPVLTAM GLLALLDEEQ
LWAEVSQAGT VLDSNHTVGV LASAHRPQGP ADAWRAAVLI YASDDTRAHP
NRSVAVTLRL RGVPPGPGLV YVTRYLDNGL CSPDGEWRRL GRPVFPTAEQ
FRRMRAAEDP VAAAPRPLPA GGRLTLRPAL RLPSLLLVHV CARPEKPPGQ
VTRLRALPLT QGQLVLVWSD EHVGSKCLWT YEIQFSQDGK AYTPVSRKPS
TFNLFVFSPD TGAVSGSYRV RALDYWARPG PFSDPVPYLE VPVPRGPPSP
GNP
UCSC uc003gby.5; human. [P35475-1];

Category

Human IDUA ELISA Kit

Human IDA ELISA Kit;Human MPS1 ELISA Kit;Human iduronidase, alpha-L- ELISA Kit;Human alpha-L-iduronidase ELISA Kit;