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Human IGHG4 ELISA Kit

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  • Catalog
    E019252
  • species
    Human
  • GeneIGHG4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of IGHG4. No significant cross-reactivity or interference between IGHG4 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IGHG4 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman IGHG4 ELISA Kit allows for the in vitro quantitative determination of IGHG4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical    Principle of the assay: IGHG4 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IGHG4 antibody and an IGHG4-HRP conjugate. The assay sample and buffer are incubated together with IGHG4-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IGHG4 concentration since IGHG4 from samples and IGHG4-HRP conjugate compete for the anti-IGHG4 antibody binding site. Since the number of sites is limited, as more sites are occupied by IGHG4 from the sample, fewer sites are left to bind IGHG4-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IGHG4 concentration in each sample is interpolated from this standard curve.
  • Human Ig gamma-4 chain C region Protein information
  • Uniprot ID IGHG4_HUMAN
  • Uniprot AC P01861;
  • UniGene Hs.510635;
  • Human Ig gamma-4 chain C region Protein SEQUENCE

  • SEQUENCE 327 AA; 35941 MW; 3EDBD811EF208E7A CRC64;

    ASTKGPSVFP LAPCSRSTSE STAALGCLVK DYFPEPVTVS WNSGALTSGV

    HTFPAVLQSS GLYSLSSVVT VPSSSLGTKT YTCNVDHKPS NTKVDKRVES

    KYGPPCPSCP APEFLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSQED

    PEVQFNWYVD GVEVHNAKTK PREEQFNSTY RVVSVLTVLH QDWLNGKEYK

    CKVSNKGLPS SIEKTISKAK GQPREPQVYT LPPSQEEMTK NQVSLTCLVK

    GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSRL TVDKSRWQEG

    NVFSCSVMHE ALHNHYTQKS LSLSLGK

  • UCSC uc059gcs.1; human.;



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