Human IGFBP1 ELISA Kit

Alternative name
Human IBP-1 ELISA Kit;Human IGF-binding protein 1 ELISA Kit;Human IGFBP-1 ELISA Kit;Human placental protein 12 ELISA Kit;Human PP12 ELISA Kit;Human IBP1 ELISA Kit;Human AFBP ELISA Kit;Human IGF-BP25 ELISA Kit;Human hIGFBP-1 ELISA Kit;Human insulin like growth factor binding protein 1 ELISA Kit;Human insulin-like growth factor-binding protein 1 ELISA Kit;Human alpha-pregnancy-associated endometrial globulin ELISA Kit;Human amniotic fluid binding protein ELISA Kit;Human binding protein-25 ELISA Kit;Human binding protein-26 ELISA Kit;Human binding protein-28 ELISA Kit;Human growth hormone independent-binding protein ELISA Kit;
Catalog
E019280
species
Human
GeneIGFBP1
SpecificityNatural and recombinant human IGFBP-1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 1pg/ml
Intended UseHuman IGFBP1 ELISA Kit allows for the in vitro quantitative determination of IGFBP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IGFBP-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-1 has been precoated onto 96-well plates. Standards(NSO,A26-N259) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP-1 amount of sample captured in plate. Background: IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesizedby human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II.3 The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copygene, located on chromosome 7.5 The standard product used in this kit is recombinant human IGFBP-1, consistingof 234 amino acids with the molecular mass of 25KDa..
Human Insulin-like growth factor-binding protein 1 Protein information
Uniprot ID IBP1_HUMAN
Uniprot AC P08833; A4D2F4; D3DVL9; Q8IYP5;
UniGene Hs.642938;
GeneID 3484
KEGG hsa:3484;
Human Insulin-like growth factor-binding protein 1 Protein SEQUENCE
SEQUENCE 259 AA; 27904 MW; 8AA75AF7DC966012 CRC64;
MSEVPVARVW LVLLLLTVQV GVTAGAPWQC APCSAEKLAL CPPVSASCSE
VTRSAGCGCC PMCALPLGAA CGVATARCAR GLSCRALPGE QQPLHALTRG
QGACVQESDA SAPHAAEAGS PESPESTEIT EEELLDNFHL MAPSEEDHSI
LWDAISTYDG SKALHVTNIK KWKEPCRIEL YRVVESLAKA QETSGEEISK
FYLPNCNKNG FYHSRQCETS MDGEAGLCWC VYPWNGKRIP GSPEIRGDPN
CQIYFNVQN
UCSC uc003tnp.4; human.;

Category

Human IGFBP1 ELISA Kit