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Human IGFBP3 ELISA Kit

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  • Alternative name

    Human IBP-3 ELISA Kit;Human IGF-binding protein 3 ELISA Kit;Human IGFBP-3 ELISA Kit;Human IBP3 ELISA Kit;Human BP-53 ELISA Kit;Human insulin like growth factor binding protein 3 ELISA Kit;Human insulin-like growth factor-binding protein 3 ELISA Kit;Human acid stable subunit of the 140 K IGF complex ELISA Kit;Human binding protein 29 ELISA Kit;Human binding protein 53 ELISA Kit;Human growth hormone-dependent binding protein ELISA Kit;
  • Catalog
    E019285
  • species
    Human
  • GeneIGFBP3
  • SpecificityNatural and recombinant human IGFBP-3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman IGFBP3 ELISA Kit allows for the in vitro quantitative determination of IGFBP3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human IGFBP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGFBP-3 has been precoated onto 96-well plates. Standards(NSO,A28Q-291) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGFBP-3 amount of sample captured in plate. Background: Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and that antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 gene (IGFBP3) expression.1Insulin-like growth factor-binding protein (IGFBP)-3, well characterized as the carrier of insulin-like growth factor (IGF), has been reported to have intrinsic bioactivity that is independent of IGF binding. IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated but not in terminally differentiated chondrocytes.2 IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent ofIGF action and are mediated through specific. IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extrIGFBP-3llular matrix.3 IGFBP3 is also located on chromosome 7.4 The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa.
  • Human Insulin-like growth factor-binding protein 3 Protein information
  • Uniprot ID IBP3_HUMAN
  • Uniprot AC P17936; A4D2F5; D3DVM0; Q2V509; Q6P1M6; Q9UCL4;
  • UniGene Hs.450230;
  • GeneID 3486
  • KEGG hsa:3486;
  • Human Insulin-like growth factor-binding protein 3 Protein SEQUENCE

  • SEQUENCE 291 AA; 31674 MW; A9682065AB266586 CRC64;

    MQRARPTLWA AALTLLVLLR GPPVARAGAS SAGLGPVVRC EPCDARALAQ

    CAPPPAVCAE LVREPGCGCC LTCALSEGQP CGIYTERCGS GLRCQPSPDE

    ARPLQALLDG RGLCVNASAV SRLRAYLLPA PPAPGNASES EEDRSAGSVE

    SPSVSSTHRV SDPKFHPLHS KIIIIKKGHA KDSQRYKVDY ESQSTDTQNF

    SSESKRETEY GPCRREMEDT LNHLKFLNVL SPRGVHIPNC DKKGFYKKKQ

    CRPSKGRKRG FCWCVDKYGQ PLPGYTTKGK EDVHCYSMQS K

  • UCSC uc003tnr.4; human. [P17936-1];



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