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Human IL1B ELISA Kit

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  • Alternative name

    Human IL-1 beta ELISA Kit;Human catabolin ELISA Kit;Human IL1F2 ELISA Kit;Human IL-1 ELISA Kit;Human IL1-BETA ELISA Kit;Human interleukin 1 beta ELISA Kit;Human interleukin-1 beta ELISA Kit;Human preinterleukin 1 beta ELISA Kit;Human pro-interleukin-1-beta ELISA Kit;
  • Catalog
    E019322
  • species
    Human
  • GeneIL1B
  • SpecificityNatural and recombinant human IL-1beta
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.15pg/ml
  • Intended UseHuman IL1B ELISA Kit allows for the in vitro quantitative determination of IL1B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human IL-1beta ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-1beta has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-1beta is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-1beta amount of sample captured in plate. Background: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting inderepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore,Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotypemodulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may beexplained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion5.
  • Human Interleukin-1 beta Protein information
  • Uniprot ID IL1B_HUMAN
  • Uniprot AC P01584; Q53X59; Q53XX2; Q7M4S7; Q7RU01; Q96HE5; Q9UCT6;
  • UniGene Hs.126256;
  • GeneID 3553
  • KEGG hsa:3553;
  • Human Interleukin-1 beta Protein SEQUENCE

  • SEQUENCE 269 AA; 30748 MW; 9BF73C3673C6FD66 CRC64;

    MAEVPELASE MMAYYSGNED DLFFEADGPK QMKCSFQDLD LCPLDGGIQL

    RISDHHYSKG FRQAASVVVA MDKLRKMLVP CPQTFQENDL STFFPFIFEE

    EPIFFDTWDN EAYVHDAPVR SLNCTLRDSQ QKSLVMSGPY ELKALHLQGQ

    DMEQQVVFSM SFVQGEESND KIPVALGLKE KNLYLSCVLK DDKPTLQLES

    VDPKNYPKKK MEKRFVFNKI EINNKLEFES AQFPNWYIST SQAENMPVFL

    GGTKGGQDIT DFTMQFVSS

  • UCSC uc002tii.2; human.;



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