Human IL11 ELISA Kit

Alternative name
Human IL-11 ELISA Kit;Human adipogenesis inhibitory factor ELISA Kit;Human AGIF ELISA Kit;Human interleukin 11 ELISA Kit;Human interleukin-11 ELISA Kit;Human oprelvekin ELISA Kit;
Catalog
E019340
species
Human
GeneIL11
SpecificityNatural and recombinant human IL-11
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman IL11 ELISA Kit allows for the in vitro quantitative determination of IL11 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-11 has been precoated onto 96-well plates. Standards(E.coli, P22-L199) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-11 amount of sample captured in plate. Background: Interleukin 11 (IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.
Human Interleukin-11 Protein information
Uniprot ID IL11_HUMAN
Uniprot AC P20809; B4DQV5; Q96EB4;
UniGene Hs.467304;
GeneID 3589
KEGG hsa:3589;
Human Interleukin-11 Protein SEQUENCE
SEQUENCE 199 AA; 21429 MW; F292BF8B34F4096D CRC64;
MNCVCRLVLV VLSLWPDTAV APGPPPGPPR VSPDPRAELD STVLLTRSLL
ADTRQLAAQL RDKFPADGDH NLDSLPTLAM SAGALGALQL PGVLTRLRAD
LLSYLRHVQW LRRAGGSSLK TLEPELGTLQ ARLDRLLRRL QLLMSRLALP
QPPPDPPAPP LAPPSSAWGG IRAAHAILGG LHLTLDWAVR GLLLLKTRL
UCSC uc002qks.3; human. [P20809-1];

Category

Human IL11 ELISA Kit

Human IL-11 ELISA Kit;Human adipogenesis inhibitory factor ELISA Kit;Human AGIF ELISA Kit;Human interleukin 11 ELISA Kit;Human interleukin-11 ELISA Kit;Human oprelvekin ELISA Kit;