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Human IL16 ELISA Kit

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  • Alternative name

    Human IL-16 ELISA Kit;Human lymphocyte chemoattractant factor ELISA Kit;Human LCF ELISA Kit;Human NIL16 ELISA Kit;Human PRIL16 ELISA Kit;Human prIL-16 ELISA Kit;Human interleukin 16 ELISA Kit;Human pro-interleukin-16 ELISA Kit;Human neuronal interleukin 16 ELISA Kit;Human prointerleukin 16 ELISA Kit;
  • Catalog
    E019370
  • species
    Human
  • GeneIL16
  • SpecificityNatural and recombinant human IL-16
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 1pg/ml
  • Intended UseHuman IL16 ELISA Kit allows for the in vitro quantitative determination of IL16 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human IL-16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-16 has been precoated onto 96-well plates. Standards(E.coli, P2-S130) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-16 amount of sample captured in plate. Background: Interleukin 16 (IL-16) is a cytokine that released by a variety of cells (including lymphocytes and some epithelial cells) that has been characterized as a chemoattractant for certain immune cells expressing the cell surface molecule CD4. By Southern blot analysis and PCR using a human/rodent somatic cell hybrid mapping panel, The human IL16 is encoded by a single-copy gene on chromosome 15. Using a combination of STS-content mapping, radiation-hybrid mapping, and genetic mapping, it was refined the assignment to 15q26.1. The mouse Il16 gene was mapped to chromosome 7 in a region showing homology of synteny to human 15q26.1. IL-16 was originally described as a factor that could attract activated T cells in humans, it was previously called lymphocyte chemoattractant factor (LCF), and the augmentation of IL16stimulation by CCR5 plays a role in regulation of Th1 cell recruitment and activation at sites of inflammation.
  • Human Interleukin-16 Protein information
  • Uniprot ID IL16_HUMAN
  • Uniprot AC Q6ZMQ7; Q9UP18;
  • UniGene Hs.459095;
  • GeneID 3603
  • KEGG hsa:3603;
  • Human Interleukin-16 Protein SEQUENCE

  • SEQUENCE 1332 AA; 141752 MW; EDF68567B2AF120C CRC64;

    MESHSRAGKS RKSAKFRSIS RSLMLCNAKT SDDGSSPDEK YPDPFEISLA

    QGKEGIFHSS VQLADTSEAG PSSVPDLALA SEAAQLQAAG NDRGKTCRRI

    FFMKESSTAS SREKPGKLEA QSSNFLFPKA CHQRARSNST SVNPYCTREI

    DFPMTKKSAA PTDRQPYSLC SNRKSLSQQL DCPAGKAAGT SRPTRSLSTA

    QLVQPSGGLQ ASVISNIVLM KGQAKGLGFS IVGGKDSIYG PIGIYVKTIF

    AGGAAAADGR LQEGDEILEL NGESMAGLTH QDALQKFKQA KKGLLTLTVR

    TRLTAPPSLC SHLSPPLCRS LSSSTCITKD SSSFALESPS APISTAKPNY

    RIMVEVSLQK EAGVGLGIGL CSVPYFQCIS GIFVHTLSPG SVAHLDGRLR

    CGDEIVEISD SPVHCLTLNE VYTILSHCDP GPVPIIVSRH PDPQVSEQQL

    KEAVAQAVEN TKFGKERHQW SLEGVKRLES SWHGRPTLEK EREKNSAPPH

    RRAQKVMIRS SSDSSYMSGS PGGSPGSGSA EKPSSDVDIS THSPSLPLAR

    EPVVLSIASS RLPQESPPLP ESRDSHPPLR LKKSFEILVR KPMSSKPKPP

    PRKYFKSDSD PQKSLEEREN SSCSSGHTPP TCGQEARELL PLLLPQEDTA

    GRSPSASAGC PGPGIGPQTK SSTEGEPGWR RASPVTQTSP IKHPLLKRQA

    RMDYSFDTTA EDPWVRISDC IKNLFSPIMS ENHGHMPLQP NASLNEEEGT

    QGHPDGTPPK LDTANGTPKV YKSADSSTVK KGPPVAPKPA WFRQSLKGLR

    NRASDPRGLP DPALSTQPAP ASREHLGSHI RASSSSSSIR QRISSFETFG

    SSQLPDKGAQ RLSLQPSSGE AAKPLGKHEE GRFSGLLGRG AAPTLVPQQP

    EQVLSSGSPA ASEARDPGVS ESPPPGRQPN QKTLPPGPDP LLRLLSTQAE

    ESQGPVLKMP SQRARSFPLT RSQSCETKLL DEKTSKLYSI SSQVSSAVMK

    SLLCLPSSIS CAQTPCIPKE GASPTSSSNE DSAANGSAET SALDTGFSLN

    LSELREYTEG LTEAKEDDDG DHSSLQSGQS VISLLSSEEL KKLIEEVKVL

    DEATLKQLDG IHVTILHKEE GAGLGFSLAG GADLENKVIT VHRVFPNGLA

    SQEGTIQKGN EVLSINGKSL KGTTHHDALA ILRQAREPRQ AVIVTRKLTP

    EAMPDLNSST DSAASASAAS DVSVESTAEA TVCTVTLEKM SAGLGFSLEG

    GKGSLHGDKP LTINRIFKGA ASEQSETVQP GDEILQLGGT AMQGLTRFEA

    WNIIKALPDG PVTIVIRRKS LQSKETTAAG DS

  • UCSC uc002bgg.4; human. [Q14005-1];



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