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Human IL33 ELISA Kit

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  • Alternative name

    Human IL-33 ELISA Kit;Human interleukin-1 family member 11 ELISA Kit;Human IL-1F11 ELISA Kit;Human nuclear factor from high endothelial venules ELISA Kit;Human NF-HEV ELISA Kit;Human C9orf26 ELISA Kit;Human IL1F11 ELISA Kit;Human NFHEV ELISA Kit;Human DVS27 ELISA Kit;Human NFEHEV ELISA Kit;Human interleukin 33 ELISA Kit;Human interleukin-33 ELISA Kit;Human DVS27-related protein ELISA Kit;Human nuclear factor for high endothelial venules ELISA Kit;
  • Catalog
    E019473
  • species
    Human
  • GeneIL33
  • SpecificityNatural and recombinant human IL-33
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman IL33 ELISA Kit allows for the in vitro quantitative determination of IL33 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: human IL-33 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-33 has been precoated onto 96-well plates. Standards(E.coli, S112-T270) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-33 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-33 amount of sample captured in plate. Background: Interleukin 33 (IL-33) is a cytokine belonging to the IL-1 superfamily. The IL33 gene maps to chromosome 9p24.1 by using of genomic database analysis. Recombinant mature human IL33 bound to ST2. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL-33. IL33, an alarmin released from necrotic cells, is necessary for potent CD8 + T cell (CTL) responses to replicating, prototypic RNA, and DNA viruses in mice. IL33 prevented the downregulation of CXCR2 and inhibition of chemotaxis induced by activation of TLR4, and found that IL33 reverses the TLR4-induced reduction of CXCR2 expression via the inhibition of expression of GRK2.
  • Human Interleukin-33 (109-270) Protein information
  • Uniprot ID IL33_HUMAN
  • Uniprot AC O95760; B2R8L1; B4DJ35; B4E1Q9; D3DRI5; E7EAX4; Q2YEJ5;
  • UniGene Hs.731660;
  • GeneID 90865
  • KEGG hsa:90865;
  • Human Interleukin-33 (109-270) Protein SEQUENCE

  • SEQUENCE 270 AA; 30759 MW; 7C158069196EF636 CRC64;

    MKPKMKYSTN KISTAKWKNT ASKALCFKLG KSQQKAKEVC PMYFMKLRSG

    LMIKKEACYF RRETTKRPSL KTGRKHKRHL VLAACQQQST VECFAFGISG

    VQKYTRALHD SSITGISPIT EYLASLSTYN DQSITFALED ESYEIYVEDL

    KKDEKKDKVL LSYYESQHPS NESGDGVDGK MLMVTLSPTK DFWLHANNKE

    HSVELHKCEK PLPDQAFFVL HNMHSNCVSF ECKTDPGVFI GVKDNHLALI

    KVDSSENLCT ENILFKLSET

  • UCSC uc011lmh.3; human. [O95760-1];



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