Human IL6R ELISA Kit

Alternative name
Human IL-6 receptor subunit alpha ELISA Kit;Human IL-6R subunit alpha ELISA Kit;Human IL-6R-alpha ELISA Kit;Human IL-6RA ELISA Kit;Human IL-6R 1 ELISA Kit;Human membrane glycoprotein 80 ELISA Kit;Human gp80 ELISA Kit;Human CD126 ELISA Kit;Human IL-6R-1 ELISA Kit;Human IL6Q ELISA Kit;Human IL6RA ELISA Kit;Human IL6RQ ELISA Kit;Human interleukin 6 receptor ELISA Kit;Human interleukin-6 receptor subunit alpha ELISA Kit;Human CD126 antigen ELISA Kit;
Catalog
E019510
species
Human
GeneIL6R
SpecificityNatural and recombinant human IL-6R Alpha
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman IL6R ELISA Kit allows for the in vitro quantitative determination of IL6R , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human IL-6R Alpha ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-6R Alpha has been precoated onto 96-well plates. Standards(sf21,L20-D358) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-6R Alpha is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-6R Alpha amount of sample captured in plate. Background: IL6R alpha(IL6RA) also known as CD126 or IL6R, is a type I cytokine receptor. The IL6 receptor is a protein complex consisting of a IL-6 receptor subunit (IL6R) and interleukin 6 signal transducer Glycoprotein 130. IL6R also denotes the human gene encoding this subunit. Alternatively spliced transcript variants encoding distinct isoforms have been reported. IL6R subunit is also shared by many other cytokines. Interleukin-6 receptor has been shown to interact with Interleukin 6 and Ciliary neurotrophic factor. Ligand binding did not appear to affect IL6R dimerization status. IL6R dimerization occurs both on the cell surface and in solution.
Human Interleukin-6 receptor subunit alpha Protein information
Uniprot ID IL6RA_HUMAN
Uniprot AC P08887; A8KAE8; B2R6V4; Q16202; Q53EQ7; Q5FWG2; Q5VZ23;
UniGene Hs.135087;
GeneID 3570
KEGG hsa:3570;
Human Interleukin-6 receptor subunit alpha Protein SEQUENCE
SEQUENCE 468 AA; 51548 MW; 62AA239FA14F1B8B CRC64;
MLAVGCALLA ALLAAPGAAL APRRCPAQEV ARGVLTSLPG DSVTLTCPGV
EPEDNATVHW VLRKPAAGSH PSRWAGMGRR LLLRSVQLHD SGNYSCYRAG
RPAGTVHLLV DVPPEEPQLS CFRKSPLSNV VCEWGPRSTP SLTTKAVLLV
RKFQNSPAED FQEPCQYSQE SQKFSCQLAV PEGDSSFYIV SMCVASSVGS
KFSKTQTFQG CGILQPDPPA NITVTAVARN PRWLSVTWQD PHSWNSSFYR
LRFELRYRAE RSKTFTTWMV KDLQHHCVIH DAWSGLRHVV QLRAQEEFGQ
GEWSEWSPEA MGTPWTESRS PPAENEVSTP MQALTTNKDD DNILFRDSAN
ATSLPVQDSS SVPLPTFLVA GGSLAFGTLL CIAIVLRFKK TWKLRALKEG
KTSMHPPYSL GQLVPERPRP TPVLVPLISP PVSPSSLGSD NTSSHNRPDA
RDPRSPYDIS NTDYFFPR
UCSC uc001fez.2; human. [P08887-1];

Category

Human IL6R ELISA Kit