Human IHH ELISA Kit

Alternative name
Human IHH ELISA Kit;Human HHG-2 ELISA Kit;Human BDA1 ELISA Kit;Human HHG2 ELISA Kit;Human indian hedgehog ELISA Kit;Human indian hedgehog protein ELISA Kit;Human Indian hedgehog homolog ELISA Kit;
Catalog
E019693
species
Human
GeneIHH
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human IHH. No significant cross-reactivity or interference between Human IHH and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.39 pg/ml
Intended UseHuman IHH ELISA Kit allows for the in vitro quantitative determination of IHH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Indian hedgehog homolog (Drosophila), also known as IHH, is a protein which is encoded by the IHH gene. The Indian Hedgehog protein is one of three proteins in the mammalian hedgehog family, the others being desert hedgehog (DHH) and Sonic hedgehog (SHH). It is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IHH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IHH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IHH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IHH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Indian hedgehog protein C-product Protein information
Uniprot ID IHH_HUMAN
Uniprot AC Q14623; B9EGM5; O43322; Q8N4B9;
UniGene Hs.654504;
GeneID 3549
KEGG hsa:3549;
Human Indian hedgehog protein C-product Protein SEQUENCE
SEQUENCE 411 AA; 45251 MW; 4DA90C83F5ABF758 CRC64;
MSPARLRPRL HFCLVLLLLL VVPAAWGCGP GRVVGSRRRP PRKLVPLAYK
QFSPNVPEKT LGASGRYEGK IARSSERFKE LTPNYNPDII FKDEENTGAD
RLMTQRCKDR LNSLAISVMN QWPGVKLRVT EGWDEDGHHS EESLHYEGRA
VDITTSDRDR NKYGLLARLA VEAGFDWVYY ESKAHVHCSV KSEHSAAAKT
GGCFPAGAQV RLESGARVAL SAVRPGDRVL AMGEDGSPTF SDVLIFLDRE
PHRLRAFQVI ETQDPPRRLA LTPAHLLFTA DNHTEPAARF RATFASHVQP
GQYVLVAGVP GLQPARVAAV STHVALGAYA PLTKHGTLVV EDVVASCFAA
VADHHLAQLA FWPLRLFHSL AWGSWTPGEG VHWYPQLLYR LGRLLLEEGS
FHPLGMSGAG S
UCSC uc002vjo.3; human.;

Category

Human IHH ELISA Kit

Human IHH ELISA Kit;Human HHG-2 ELISA Kit;Human BDA1 ELISA Kit;Human HHG2 ELISA Kit;Human indian hedgehog ELISA Kit;Human indian hedgehog protein ELISA Kit;Human Indian hedgehog homolog ELISA Kit;