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Human IKBKB ELISA Kit

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  • Alternative name

    Human I-kappa-B-kinase beta ELISA Kit;Human IKK-B ELISA Kit;Human IKK-beta ELISA Kit;Human IkBKB ELISA Kit;Human I-kappa-B kinase 2 ELISA Kit;Human IKK2 ELISA Kit;Human nuclear factor NF-kappa-B inhibitor kinase beta ELISA Kit;Human NFKBIKB ELISA Kit;Human IKKB ELISA Kit;Human IMD15 ELISA Kit;Human inhibitor of nuclear factor kappa B kinase subunit beta ELISA Kit;Human inhibitor of nuclear factor kappa-B kinase subunit beta ELISA Kit;Human inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta ELISA Kit;
  • Catalog
    E019819
  • species
    Human
  • GeneIKBKB
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of IkBKb. No significant cross-reactivity or interference between IkBKb and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of IkBKb is typically less than 0.059ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
  • Intended UseHuman IKBKB ELISA Kit allows for the in vitro quantitative determination of IKBKB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IkBKb. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IkBKb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IkBKb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IkBKb in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Inhibitor of nuclear factor kappa-B kinase subunit beta Protein information
  • Uniprot ID IKKB_HUMAN
  • Uniprot AC SKVRGPVS GSPDSMNASR LSQPGQLMSQ PSTASNSLPE PAKKSEELVA EAHNLCTLLE
  • UniGene Hs.597664;
  • GeneID 3551
  • KEGG hsa:3551;
  • Human Inhibitor of nuclear factor kappa-B kinase subunit beta Protein SEQUENCE

  • SEQUENCE 756 AA; 86564 MW; F9CADF671AE9E14E CRC64;

    MSWSPSLTTQ TCGAWEMKER LGTGGFGNVI RWHNQETGEQ IAIKQCRQEL

    SPRNRERWCL EIQIMRRLTH PNVVAARDVP EGMQNLAPND LPLLAMEYCQ

    GGDLRKYLNQ FENCCGLREG AILTLLSDIA SALRYLHENR IIHRDLKPEN

    IVLQQGEQRL IHKIIDLGYA KELDQGSLCT SFVGTLQYLA PELLEQQKYT

    VTVDYWSFGT LAFECITGFR PFLPNWQPVQ WHSKVRQKSE VDIVVSEDLN

    GTVKFSSSLP YPNNLNSVLA ERLEKWLQLM LMWHPRQRGT DPTYGPNGCF

    KALDDILNLK LVHILNMVTG TIHTYPVTED ESLQSLKARI QQDTGIPEED

    QELLQEAGLA LIPDKPATQC ISDGKLNEGH TLDMDLVFLF DNSKITYETQ

    ISPRPQPESV SCILQEPKRN LAFFQLRKVW GQVWHSIQTL KEDCNRLQQG

    QRAAMMNLLR NNSCLSKMKN SMASMSQQLK AKLDFFKTSI QIDLEKYSEQ

    TEFGITSDKL LLAWREMEQA VELCGRENEV KLLVERMMAL QTDIVDLQRS

    PMGRKQGGTL DDLEEQAREL YRRLREKPRD QRTEGDSQEM VRLLLQAIQS

    FEKKVRVIYT QLSKTVVCKQ KALELLPKVE EVVSLMNEDE KTVVRLQEKR

    QKELWNLLKI ACSKVRGPVS GSPDSMNASR LSQPGQLMSQ PSTASNSLPE

    PAKKSEELVA EAHNLCTLLE NAIQDTVREQ DQSFTALDWS WLQTEEEEHS

    CLEQAS

  • UCSC uc003xow.3; human. [O14920-1];



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