Human PPA1 ELISA Kit

Alternative name
Human pyrophosphate phospho-hydrolase ELISA Kit;Human PPase ELISA Kit;Human IOPPP ELISA Kit;Human PP ELISA Kit;Human HEL-S-66p ELISA Kit;Human PP1 ELISA Kit;Human SID6-8061 ELISA Kit;Human pyrophosphatase (inorganic) 1 ELISA Kit;Human inorganic pyrophosphatase ELISA Kit;Human cytosolic inorganic pyrophosphatase ELISA Kit;Human diphosphate phosphohydrolase ELISA Kit;Human epididymis secretory sperm binding protein Li 66p ELISA Kit;Human inorganic diphosphatase 1 ELISA Kit;Human inorganic pyrophosphatase 1 ELISA Kit;Human pyrophosphatase 1 ELISA Kit;
Catalog
E019843
species
Human
GenePPA1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 9.4pg/ml
Intended UseHuman PPA1 ELISA Kit allows for the in vitro quantitative determination of PPA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human PPA1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human PPA1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human PPA1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human PPA1 can be calculated.
Human Inorganic pyrophosphatase Protein information
Uniprot ID IPYR_HUMAN
Uniprot AC Q15181; Q2M348; Q5SQT7; Q6P7P4; Q9UQJ5; Q9Y5B1;
UniGene Hs.437403;
GeneID 5464
KEGG hsa:5464;
Human Inorganic pyrophosphatase Protein SEQUENCE
SEQUENCE 289 AA; 32660 MW; E3973C9E6F8CA5CD CRC64;
MSGFSTEERA APFSLEYRVF LKNEKGQYIS PFHDIPIYAD KDVFHMVVEV
PRWSNAKMEI ATKDPLNPIK QDVKKGKLRY VANLFPYKGY IWNYGAIPQT
WEDPGHNDKH TGCCGDNDPI DVCEIGSKVC ARGEIIGVKV LGILAMIDEG
ETDWKVIAIN VDDPDAANYN DINDVKRLKP GYLEATVDWF RRYKVPDGKP
ENEFAFNAEF KDKDFAIDII KSTHDHWKAL VTKKTNGKGI SCMNTTLSES
PFKCDPDAAR AIVDALPPPC ESACTVPTDV DKWFHHQKN

Category

Human PPA1 ELISA Kit