Human ITPA ELISA Kit

Alternative name
Human ITPase ELISA Kit;Human inosine triphosphatase ELISA Kit;Human non-canonical purine NTP pyrophosphatase ELISA Kit;Human non-standard purine NTP pyrophosphatase ELISA Kit;Human nucleoside-triphosphate diphosphatase ELISA Kit;Human Nucleoside-triphosphate pyrophosphatase ELISA Kit;Human NTPase ELISA Kit;Human putative oncogene protein HLC14-06-P ELISA Kit;Human C20orf37 ELISA Kit;Human HLC14-06-P ELISA Kit;Human My049 ELISA Kit;Human dJ794I6.3 ELISA Kit;Human inosine triphosphate pyrophosphatase ELISA Kit;Human inosine triphosphatase (nucleoside triphosphate pyrophosphatase) ELISA Kit;Human inosine triphosphate pyrophosphohydrolase ELISA Kit;
Catalog
E019850
species
Human
GeneITPA
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.469ng/ml
Intended UseHuman ITPA ELISA Kit allows for the in vitro quantitative determination of ITPA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ITPA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ITPA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ITPA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ITPA can be calculated.
Human Inosine triphosphate pyrophosphatase Protein information
Uniprot ID ITPA_HUMAN
Uniprot AC Q9H3H8;
UniGene Hs.415299;
GeneID 3704
KEGG hsa:3704;
Human Inosine triphosphate pyrophosphatase Protein SEQUENCE
SEQUENCE 194 AA; 21446 MW; F0EC6A523722DF05 CRC64;
MAASLVGKKI VFVTGNAKKL EEVVQILGDK FPCTLVAQKI DLPEYQGEPD
EISIQKCQEA VRQVQGPVLV EDTCLCFNAL GGLPGPYIKW FLEKLKPEGL
HQLLAGFEDK SAYALCTFAL STGDPSQPVR LFRGRTSGRI VAPRGCQDFG
WDPCFQPDGY EQTYAEMPKA EKNAVSHRFR ALLELQEYFG SLAA
UCSC uc002wid.4; human. [Q9BY32-1];

Category

Human ITPA ELISA Kit