Human IDE ELISA Kit

Alternative name
Human Abeta-degrading protease ELISA Kit;Human insulin protease ELISA Kit;Human insulinase ELISA Kit;Human INSULYSIN ELISA Kit;Human insulin degrading enzyme ELISA Kit;Human insulin-degrading enzyme ELISA Kit;
Catalog
E019917
species
Human
GeneIDE
SpecificityThis assay has high sensitivity and excellent specificity for detection of IDE. No significant cross-reactivity or interference between IDE and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.138ng/mL.
Intended UseHuman IDE ELISA Kit allows for the in vitro quantitative determination of IDE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IDE in human serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to IDE. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IDE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IDE, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IDE in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Insulin-degrading enzyme Protein information
Uniprot ID IDE_HUMAN
Uniprot AC P14735; B2R721; B7ZAU2; D3DR35; Q5T5N2;
UniGene Hs.500546;
GeneID 3416
KEGG hsa:3416;
Human Insulin-degrading enzyme Protein SEQUENCE
SEQUENCE 1019 AA; 117968 MW; 8A28AEF75EDA0EDA CRC64;
MRYRLAWLLH PALPSTFRSV LGARLPPPER LCGFQKKTYS KMNNPAIKRI
GNHITKSPED KREYRGLELA NGIKVLLISD PTTDKSSAAL DVHIGSLSDP
PNIAGLSHFC EHMLFLGTKK YPKENEYSQF LSEHAGSSNA FTSGEHTNYY
FDVSHEHLEG ALDRFAQFFL CPLFDESCKD REVNAVDSEH EKNVMNDAWR
LFQLEKATGN PKHPFSKFGT GNKYTLETRP NQEGIDVRQE LLKFHSAYYS
SNLMAVCVLG RESLDDLTNL VVKLFSEVEN KNVPLPEFPE HPFQEEHLKQ
LYKIVPIKDI RNLYVTFPIP DLQKYYKSNP GHYLGHLIGH EGPGSLLSEL
KSKGWVNTLV GGQKEGARGF MFFIINVDLT EEGLLHVEDI ILHMFQYIQK
LRAEGPQEWV FQECKDLNAV AFRFKDKERP RGYTSKIAGI LHYYPLEEVL
TAEYLLEEFR PDLIEMVLDK LRPENVRVAI VSKSFEGKTD RTEEWYGTQY
KQEAIPDEVI KKWQNADLNG KFKLPTKNEF IPTNFEILPL EKEATPYPAL
IKDTAMSKLW FKQDDKFFLP KACLNFEFFS PFAYVDPLHC NMAYLYLELL
KDSLNEYAYA AELAGLSYDL QNTIYGMYLS VKGYNDKQPI LLKKIIEKMA
TFEIDEKRFE IIKEAYMRSL NNFRAEQPHQ HAMYYLRLLM TEVAWTKDEL
KEALDDVTLP RLKAFIPQLL SRLHIEALLH GNITKQAALG IMQMVEDTLI
EHAHTKPLLP SQLVRYREVQ LPDRGWFVYQ QRNEVHNNCG IEIYYQTDMQ
STSENMFLEL FCQIISEPCF NTLRTKEQLG YIVFSGPRRA NGIQGLRFII
QSEKPPHYLE SRVEAFLITM EKSIEDMTEE AFQKHIQALA IRRLDKPKKL
SAECAKYWGE IISQQYNFDR DNTEVAYLKT LTKEDIIKFY KEMLAVDAPR
RHKVSVHVLA REMDSCPVVG EFPCQNDINL SQAPALPQPE VIQNMTEFKR
GLPLFPLVKP HINFMAAKL
UCSC uc001kia.4; human. [P14735-1];

Category

Human IDE ELISA Kit

Human Abeta-degrading protease ELISA Kit;Human insulin protease ELISA Kit;Human insulinase ELISA Kit;Human INSULYSIN ELISA Kit;Human insulin degrading enzyme ELISA Kit;Human insulin-degrading enzyme ELISA Kit;