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Alternative name
Insulin receptor substrate 1 ELISA KIT; IRS1 ELISA KIT; IRS-1 ELISA KIT
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Catalog
E019980
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species
Human
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GeneIRS-1
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SpecificityNo significant cross-reactivity or interference between Human IRS-1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity2.0pg/ml.
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Intended UseHuman IRS-1 ELISA Kit allows for the in vitro quantitative determination of IRS-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntroduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic or diagnostic applications! It is intended to be determinated IRS-1 concentrations in Human serum, plasma and other body fluids. Using Purified Human IRS-1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add IRS-1 and IRS-1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IRS-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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