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Human IRS2 ELISA Kit

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  • Alternative name

    Human IRS-2 ELISA Kit;Human insulin receptor substrate 2 ELISA Kit;
  • Catalog
    E019986
  • species
    Human
  • GeneIRS2
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of IRS2. No significant cross-reactivity or interference between IRS2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.063ng/mL.
  • Intended UseHuman IRS2 ELISA Kit allows for the in vitro quantitative determination of IRS2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IRS2 in human tissue homogenates, cell lysates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to IRS2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to IRS2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain IRS2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IRS2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Insulin receptor substrate 2 Protein information
  • Uniprot ID IRS2_HUMAN
  • Uniprot AC Q9Y4H2; Q96RR2; Q9BZG0; Q9Y6I5;
  • UniGene Hs.442344;
  • GeneID 8660
  • KEGG hsa:8660;
  • Human Insulin receptor substrate 2 Protein SEQUENCE

  • SEQUENCE 1338 AA; 137334 MW; 58E569E8BDBAF3D7 CRC64;

    MASPPRHGPP GPASGDGPNL NNNNNNNNHS VRKCGYLRKQ KHGHKRFFVL

    RGPGAGGDEA TAGGGSAPQP PRLEYYESEK KWRSKAGAPK RVIALDCCLN

    INKRADAKHK YLIALYTKDE YFAVAAENEQ EQEGWYRALT DLVSEGRAAA

    GDAPPAAAPA ASCSASLPGA LGGSAGAAGA EDSYGLVAPA TAAYREVWQV

    NLKPKGLGQS KNLTGVYRLC LSARTIGFVK LNCEQPSVTL QLMNIRRCGH

    SDSFFFIEVG RSAVTGPGEL WMQADDSVVA QNIHETILEA MKALKELFEF

    RPRSKSQSSG SSATHPISVP GARRHHHLVN LPPSQTGLVR RSRTDSLAAT

    PPAAKCSSCR VRTASEGDGG AAAGAAAAGA RPVSVAGSPL SPGPVRAPLS

    RSHTLSGGCG GRGSKVALLP AGGALQHSRS MSMPVAHSPP AATSPGSLSS

    SSGHGSGSYP PPPGPHPPLP HPLHHGPGQR PSSGSASASG SPSDPGFMSL

    DEYGSSPGDL RAFCSHRSNT PESIAETPPA RDGGGGGEFY GYMTMDRPLS

    HCGRSYRRVS GDAAQDLDRG LRKRTYSLTT PARQRPVPQP SSASLDEYTL

    MRATFSGSAG RLCPSCPASS PKVAYHPYPE DYGDIEIGSH RSSSSNLGAD

    DGYMPMTPGA ALAGSGSGSC RSDDYMPMSP ASVSAPKQIL QPRAAAAAAA

    AVPSAGPAGP APTSAAGRTF PASGGGYKAS SPAESSPEDS GYMRMWCGSK

    LSMEHADGKL LPNGDYLNVS PSDAVTTGTP PDFFSAALHP GGEPLRGVPG

    CCYSSLPRSY KAPYTCGGDS DQYVLMSSPV GRILEEERLE PQATPGPSQA

    ASAFGAGPTQ PPHPVVPSPV RPSGGRPEGF LGQRGRAVRP TRLSLEGLPS

    LPSMHEYPLP PEPKSPGEYI NIDFGEPGAR LSPPAPPLLA SAASSSSLLS

    ASSPASSLGS GTPGTSSDSR QRSPLSDYMN LDFSSPKSPK PGAPSGHPVG

    SLDGLLSPEA SSPYPPLPPR PSASPSSSLQ PPPPPPAPGE LYRLPPASAV

    ATAQGPGAAS SLSSDTGDNG DYTEMAFGVA ATPPQPIAAP PKPEAARVAS

    PTSGVKRLSL MEQVSGVEAF LQASQPPDPH RGAKVIRADP QGGRRRHSSE

    TFSSTTTVTP VSPSFAHNPK RHNSASVENV SLRKSSEGGV GVGPGGGDEP

    PTSPRQLQPA PPLAPQGRPW TPGQPGGLVG CPGSGGSPMR RETSAGFQNG

    LNYIAIDVRE EPGLPPQPQP PPPPLPQPGD KSSWGRTRSL GGLISAVGVG

    STGGGCGGPG PGALPPANTY ASIDFLSHHL KEATIVKE

  • UCSC uc001vqv.4; human.;



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