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Human ITIH4 ELISA Kit

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  • Alternative name

    Human ITI heavy chain H4 ELISA Kit;Human ITI-HC4 ELISA Kit;Human inter-alpha-inhibitor heavy chain 4 ELISA Kit;Human inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit;Human IHRP ELISA Kit;Human Plasma kallikrein sensitive glycoprotein 120 ELISA Kit;Human GP120 ELISA Kit;Human PK-120 ELISA Kit;Human ITIHL1 ELISA Kit;Human PK120 ELISA Kit;Human H4P ELISA Kit;Human inter-alpha-trypsin inhibitor heavy chain family member 4 ELISA Kit;Human inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit;Human inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein) ELISA Kit;Human inter-alpha-trypsin inhibitor, heavy chain-like, 1 ELISA Kit;Human plasma kallikrein-sensitive glycoprotein 120 ELISA Kit;
  • Catalog
    E020184
  • species
    Human
  • GeneITIH4
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Inter Alpha-Globulin Inhibitor H4 (ITIH4). No significant cross-reactivity or interference between Inter Alpha-Globulin Inhibitor H4 (ITIH4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.14ng/mL.
  • Intended UseHuman ITIH4 ELISA Kit allows for the in vitro quantitative determination of ITIH4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inter Alpha-Globulin Inhibitor H4 (ITIH4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inter Alpha-Globulin Inhibitor H4 (ITIH4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Inter Alpha-Globulin Inhibitor H4 (ITIH4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 Protein information
  • Uniprot ID ITIH4_HUMAN
  • Uniprot AC Q14624; B7Z545; E9PGN5; Q15135; Q9P190; Q9UQ54;
  • UniGene Hs.709406;
  • GeneID 3700
  • KEGG hsa:3700;
  • Human 35 kDa inter-alpha-trypsin inhibitor heavy chain H4 Protein SEQUENCE

  • SEQUENCE 930 AA; 103357 MW; 847A9A5CB886C5A4 CRC64;

    MKPPRPVRTC SKVLVLLSLL AIHQTTTAEK NGIDIYSLTV DSRVSSRFAH

    TVVTSRVVNR ANTVQEATFQ MELPKKAFIT NFSMIIDGMT YPGIIKEKAE

    AQAQYSAAVA KGKSAGLVKA TGRNMEQFQV SVSVAPNAKI TFELVYEELL

    KRRLGVYELL LKVRPQQLVK HLQMDIHIFE PQGISFLETE STFMTNQLVD

    ALTTWQNKTK AHIRFKPTLS QQQKSPEQQE TVLDGNLIIR YDVDRAISGG

    SIQIENGYFV HYFAPEGLTT MPKNVVFVID KSGSMSGRKI QQTREALIKI

    LDDLSPRDQF NLIVFSTEAT QWRPSLVPAS AENVNKARSF AAGIQALGGT

    NINDAMLMAV QLLDSSNQEE RLPEGSVSLI ILLTDGDPTV GETNPRSIQN

    NVREAVSGRY SLFCLGFGFD VSYAFLEKLA LDNGGLARRI HEDSDSALQL

    QDFYQEVANP LLTAVTFEYP SNAVEEVTQN NFRLLFKGSE MVVAGKLQDR

    GPDVLTATVS GKLPTQNITF QTESSVAEQE AEFQSPKYIF HNFMERLWAY

    LTIQQLLEQT VSASDADQQA LRNQALNLSL AYSFVTPLTS MVVTKPDDQE

    QSQVAEKPME GESRNRNVHS GSTFFKYYLQ GAKIPKPEAS FSPRRGWNRQ

    AGAAGSRMNF RPGVLSSRQL GLPGPPDVPD HAAYHPFRRL AILPASAPPA

    TSNPDPAVSR VMNMKIEETT MTTQTPAPIQ APSAILPLPG QSVERLCVDP

    RHRQGPVNLL SDPEQGVEVT GQYEREKAGF SWIEVTFKNP LVWVHASPEH

    VVVTRNRRSS AYKWKETLFS VMPGLKMTMD KTGLLLLSDP DKVTIGLLFW

    DGRGEGLRLL LRDTDRFSSH VGGTLGQFYQ EVLWGSPAAS DDGRRTLRVQ

    GNDHSATRER RLDYQEGPPG VEISCWSVEL

  • UCSC uc003dfz.4; human. [Q14624-1];



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