Human IFNA21 ELISA Kit

Alternative name
Human IFN-alpha-21 ELISA Kit;Human interferon alpha-F ELISA Kit;Human LeIF F ELISA Kit;Human IFN-alphaI ELISA Kit;Human leIF-F ELISA Kit;Human interferon alpha 21 ELISA Kit;Human interferon alpha-21 ELISA Kit;Human leukocyte interferon protein ELISA Kit;
Catalog
E020245
species
Human
GeneIFNA21
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman IFNA21 ELISA Kit allows for the in vitro quantitative determination of IFNA21 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalPrinciple of the Assay: IFNA21 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IFNA21 antibody and an IFNA21-HRP conjugate. The assay sample and buffer are incubated together with IFNA21-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNA21 concentration since IFNA21 from samples and IFNA21-HRP conjugate compete for the anti-IFNA21 antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNA21 from the sample, fewer sites are left to bind IFNA21-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNA21 concentration in each sample is interpolated from this standard curve.
Human Interferon alpha-21 Protein information
Uniprot ID IFN21_HUMAN
Uniprot AC VIQEVGVE ETPLMNVDSI LAVKKYFQRI TLYLTEKKYS PCAWEVVRAE IMRSFSLSKI
UniGene Hs.113211;
GeneID 3452
KEGG hsa:3452;
Human Interferon alpha-21 Protein SEQUENCE
SEQUENCE 189 AA; 21741 MW; F0B6C9C392905802 CRC64;
MALSFSLLMA VLVLSYKSIC SLGCDLPQTH SLGNRRALIL LAQMGRISPF
SCLKDRHDFG FPQEEFDGNQ FQKAQAISVL HEMIQQTFNL FSTKDSSATW
EQSLLEKFST ELNQQLNDLE ACVIQEVGVE ETPLMNVDSI LAVKKYFQRI
TLYLTEKKYS PCAWEVVRAE IMRSFSLSKI FQERLRRKE
UCSC uc003zom.2; human.;

Category

Human IFNA21 ELISA Kit

Human IFN-alpha-21 ELISA Kit;Human interferon alpha-F ELISA Kit;Human LeIF F ELISA Kit;Human IFN-alphaI ELISA Kit;Human leIF-F ELISA Kit;Human interferon alpha 21 ELISA Kit;Human interferon alpha-21 ELISA Kit;Human leukocyte interferon protein ELISA Kit;