Human IFNE ELISA Kit

Alternative name
Human IFN-epsilon ELISA Kit;Human Interferon epsilon-1 ELISA Kit;Human IFNE1 ELISA Kit;Human IFN-E ELISA Kit;Human IFNT1 ELISA Kit;Human INFE1 ELISA Kit;Human PRO655 ELISA Kit;Human interferon epsilon ELISA Kit;Human interferon epsilon 1 ELISA Kit;Human interferon tau-1 ELISA Kit;
Catalog
E020283
species
Human
GeneIFNE
SpecificityThis assay has high sensitivity and excellent specificity for detection of IFNepsilon. No significant cross-reactivity or interference between IFNepsilon and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IFNepsilon and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman IFNE ELISA Kit allows for the in vitro quantitative determination of IFNE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalIntended Uses: This IFNepsilon ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human IFNepsilon. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||IFNepsilon ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IFNepsilon antibody and an IFNepsilon-HRP conjugate. The assay sample and buffer are incubated together with IFNepsilon-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IFNepsilon concentration since IFNepsilon from samples and IFNepsilon-HRP conjugate compete for the anti-IFNepsilon antibody binding site. Since the number of sites is limited, as more sites are occupied by IFNepsilon from the sample, fewer sites are left to bind IFNepsilon-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNepsilon concentration in each sample is interpolated from this standard curve.
Human Interferon epsilon Protein information
Uniprot ID IFNE_HUMAN
Uniprot AC Q86WN2;
UniGene Hs.682604;
GeneID 338376
KEGG hsa:338376;
Human Interferon epsilon Protein SEQUENCE
SEQUENCE 208 AA; 24414 MW; 65FB861407F14E5F CRC64;
MIIKHFFGTV LVLLASTTIF SLDLKLIIFQ QRQVNQESLK LLNKLQTLSI
QQCLPHRKNF LLPQKSLSPQ QYQKGHTLAI LHEMLQQIFS LFRANISLDG
WEENHTEKFL IQLHQQLEYL EALMGLEAEK LSGTLGSDNL RLQVKMYFRR
IHDYLENQDY STCAWAIVQV EISRCLFFVF SLTEKLSKQG RPLNDMKQEL
TTEFRSPR
UCSC uc003zpg.4; human.;

Category

Human IFNE ELISA Kit

Human IFN-epsilon ELISA Kit;Human Interferon epsilon-1 ELISA Kit;Human IFNE1 ELISA Kit;Human IFN-E ELISA Kit;Human IFNT1 ELISA Kit;Human INFE1 ELISA Kit;Human PRO655 ELISA Kit;Human interferon epsilon ELISA Kit;Human interferon epsilon 1 ELISA Kit;Human interferon tau-1 ELISA Kit;