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Alternative name
Interleukin-1 receptor accessory protein ELISA KIT; Interleukin-1 receptor 3 ELISA KIT; IL-1R-3 ELISA KIT; IL-1R3IL1RAP ELISA KIT; C3orf13 ELISA KIT; IL1R3 ELISA KIT; IL-1 receptor accessory protein ELISA KIT; IL-1RAcP ELISA KIT; IL-1R-3 ELISA KIT; IL-1R3 ELISA KIT
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Catalog
E020430
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species
Human
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GeneIL 1Racp
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SpecificityThis assay has high sensitivity and excellent specificity for detection of IL-1Racp. No significant cross-reactivity or interference between IL-1Racp and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-1Racp and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseHuman IL 1Racp ELISA Kit allows for the in vitro quantitative determination of IL 1Racp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology
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Product Description
specificalPrinciple of the assay: IL-1Racp ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-1Racp. Standards or samples are then added to the microtiter plate wells and IL-1Racp if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-1Racp present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-1Racp are added to each well to "sandwich" the IL-1Racp immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-1Racp and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1Racp concentration in each sample is interpolated from this standard curve.
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