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Alternative name
Interleukin-1 receptor antagonist protein ELISA KIT; ICIL-1RA ELISA KIT; IL1 inhibitor ELISA KIT; INN: AnakinraIL1RN ELISA KIT; IL1F3 ELISA KIT; IL1RA ELISA KIT; IL-1RN ELISA KIT; IL-1ra ELISA KIT; IRAP ELISA KIT
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Catalog
E020439
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species
Human
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GeneIL-1ra/IL-1F3
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<18.75pg/ml
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Intended UseHuman IL-1ra/IL-1F3 ELISA Kit allows for the in vitro quantitative determination of IL-1ra/IL-1F3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human IL-1RA antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human IL-1RA antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human IL-1RA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human IL-1RA can be calculated.
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