Human IL10RB ELISA Kit

Alternative name
Human IL-10 receptor subunit beta ELISA Kit;Human IL-10R subunit beta ELISA Kit;Human IL-10RB ELISA Kit;Human cytokine receptor class-II member 4 ELISA Kit;Human cytokine receptor family 2 member 4 ELISA Kit;Human CRF2-4 ELISA Kit;Human interleukin-10 receptor subunit 2 ELISA Kit;Human IL-10R subunit 2 ELISA Kit;Human IL-10R2 ELISA Kit;Human CRFB4 ELISA Kit;Human D21S58 ELISA Kit;Human D21S66 ELISA Kit;Human CDW210B ELISA Kit;Human interleukin 10 receptor subunit beta ELISA Kit;Human interleukin-10 receptor subunit beta ELISA Kit;Human cytokine receptor class-II CRF2-4 ELISA Kit;Human cytokine receptor family II, member 4 ELISA Kit;Human interleukin 10 receptor, beta ELISA Kit;
Catalog
E020480
species
Human
GeneIL10RB
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 10 Receptor Beta (IL10Rb). No significant cross-reactivity or interference between Interleukin 10 Receptor Beta (IL10Rb) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 31pg/mL.
Intended UseHuman IL10RB ELISA Kit allows for the in vitro quantitative determination of IL10RB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 Receptor Beta (IL10Rb). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 10 Receptor Beta (IL10Rb). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 10 Receptor Beta (IL10Rb), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Interleukin 10 Receptor Beta (IL10Rb) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Interleukin-10 receptor subunit beta Protein information
Uniprot ID I10R2_HUMAN
Uniprot AC Q08334; Q9BUU4;
UniGene Hs.654593;
GeneID 3588
KEGG hsa:3588;
Human Interleukin-10 receptor subunit beta Protein SEQUENCE
SEQUENCE 325 AA; 36995 MW; E470726619AF54C2 CRC64;
MAWSLGSWLG GCLLVSALGM VPPPENVRMN SVNFKNILQW ESPAFAKGNL
TFTAQYLSYR IFQDKCMNTT LTECDFSSLS KYGDHTLRVR AEFADEHSDW
VNITFCPVDD TIIGPPGMQV EVLADSLHMR FLAPKIENEY ETWTMKNVYN
SWTYNVQYWK NGTDEKFQIT PQYDFEVLRN LEPWTTYCVQ VRGFLPDRNK
AGEWSEPVCE QTTHDETVPS WMVAVILMAS VFMVCLALLG CFALLWCVYK
KTKYAFSPRN SLPQHLKEFL GHPHHNTLLF FSFPLSDEND VFDKLSVIAE
DSESGKQNPG DSCSLGTPPG QGPQS
UCSC uc002yrk.3; human.;

Category

Human IL10RB ELISA Kit