Human IL32 ELISA Kit

Alternative name
Human IL-32 ELISA Kit;Human natural killer cells protein 4 ELISA Kit;Human tumor necrosis factor alpha-inducing factor ELISA Kit;Human NK4 ELISA Kit;Human TAIF ELISA Kit;Human IL-32alpha ELISA Kit;Human IL-32beta ELISA Kit;Human IL-32delta ELISA Kit;Human IL-32gamma ELISA Kit;Human TAIFa ELISA Kit;Human TAIFb ELISA Kit;Human TAIFc ELISA Kit;Human TAIFd ELISA Kit;Human interleukin 32 ELISA Kit;Human interleukin-32 ELISA Kit;Human interleukin-32 eta ELISA Kit;Human interleukin-32 small ELISA Kit;Human interleukin-32 theta ELISA Kit;Human natural killer cell transcript 4 ELISA Kit;
Catalog
E020711
species
Human
GeneIL32
SpecificityThis assay has high sensitivity and excellent specificity for detection of IL-32. No significant cross-reactivity or interference between IL-32 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-32 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman IL32 ELISA Kit allows for the in vitro quantitative determination of IL32 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: IL-32 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-32. Standards or samples are then added to the microtiter plate wells and IL-32 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-32 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for IL-32 are added to each well to "sandwich" the IL-32 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-32 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-32 concentration in each sample is interpolated from this standard curve.
Human Interleukin-32 Protein information
Uniprot ID IL32_HUMAN
Uniprot AC Q5VFH8; Q8WV38; Q96GK9;
UniGene Hs.943;
GeneID 9235
KEGG hsa:9235;
Human Interleukin-32 Protein SEQUENCE
SEQUENCE 234 AA; 26676 MW; 5F339FE2D348A761 CRC64;
MCFPKVLSDD MKKLKARMVM LLPTSAQGLG AWVSACDTED TVGHLGPWRD
KDPALWCQLC LSSQHQAIER FYDKMQNAES GRGQVMSSLA ELEDDFKEGY
LETVAAYYEE QHPELTPLLE KERDGLRCRG NRSPVPDVED PATEEPGESF
CDKVMRWFQA MLQRLQTWWH GVLAWVKEKV VALVHAVQAL WKQFQSFCCS
LSELFMSSFQ SYGAPRGDKE ELTPQKCSEP QSSK
UCSC uc002ctk.4; human. [P24001-1];

Category

Human IL32 ELISA Kit