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Alternative name
Interleukin-2 receptor subunit alpha ELISA KIT; p55 ELISA KIT; CD_antigen: CD25Il2ra ELISA KIT; Il2r ELISA KIT; IL-2 receptor subunit alpha ELISA KIT; IL-2-RA ELISA KIT; IL-2R subunit alpha ELISA KIT; IL2-RA ELISA KIT
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Catalog
E020885
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species
Human
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GeneIL-2R
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SpecificityThis assay has high sensitivity and excellent specificity for detection of human IL2R. No significant cross-reactivity or interference between human IL2R and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of human IL2R is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
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Intended UseHuman IL-2R ELISA Kit allows for the in vitro quantitative determination of IL-2R , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL2R has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL2R present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL2R is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL2R bound in the initial step. The color development is stopped and the intensity of the color is measured.
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