Human INA ELISA Kit

Alternative name
Human alpha-Inx ELISA Kit;Human 66 kDa neurofilament protein ELISA Kit;Human NF-66 ELISA Kit;Human Neurofilament-66 ELISA Kit;Human Neurofilament 5 ELISA Kit;Human NEF5 ELISA Kit;Human TXBP-1 ELISA Kit;Human internexin neuronal intermediate filament protein alpha ELISA Kit;Human alpha-internexin ELISA Kit;Human neurofilament 5 (66kD) ELISA Kit;Human neurofilament-66, tax-binding protein ELISA Kit;
Catalog
E020974
species
Human
GeneINA
SpecificityThis assay has high sensitivity and excellent specificity for detection of INa. No significant cross-reactivity or interference between INa and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of INa is typically less than 0.058ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
Intended UseHuman INA ELISA Kit allows for the in vitro quantitative determination of INA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to INa. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to INa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain INa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of INa in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Alpha-internexin Protein information
Uniprot ID AINX_HUMAN
Uniprot AC Q16352; B1AQK0; Q9BRC5;
UniGene Hs.500916;
GeneID 9118
KEGG hsa:9118;
Human Alpha-internexin Protein SEQUENCE
SEQUENCE 499 AA; 55391 MW; 4C972764E9E68D3E CRC64;
MSFGSEHYLC SSSSYRKVFG DGSRLSARLS GAGGAGGFRS QSLSRSNVAS
SAACSSASSL GLGLAYRRPP ASDGLDLSQA AARTNEYKII RTNEKEQLQG
LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELRDL
RAQLEEASSA RSQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ
RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA
EVDVTVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE
VAGYQDSIGQ LENDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL
EGEETRFSTS GLSISGLNPL PNPSYLLPPR ILSATTSKVS STGLSLKKEE
EEEEASKVAS KKTSQIGESF EEILEETVIS TKKTEKSNIE ETTISSQKI
UCSC uc001kws.3; human.;

Category

Human INA ELISA Kit